BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Regulation of oncogene expression in T-DNA-transformed host plant cells

Zhang, Y.; Lee, C.; Wehner, N.; Imdahl, F.; Svetlana, V.; Weiste, C.; Droege-Laser, W.; Deeken, R.; PLoS Pathog. 11, 1-27 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Agrobacterium tumefaciens
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Agrobacterium tumefaciens C58
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
Q9R717
-
-
Agrobacterium tumefaciens C58
Q9R717
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
746273
Agrobacterium tumefaciens
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
746273
Agrobacterium tumefaciens C58
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Agrobacterium tumefaciens
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Agrobacterium tumefaciens C58
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
746273
Agrobacterium tumefaciens
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
746273
Agrobacterium tumefaciens C58
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Agrobacterium tumefaciens
gene IaaM is constitutively expressed and no transcription factor further activates its promoter
additional information
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of auxin
Agrobacterium tumefaciens
physiological function
Crown gall development requires the expression of agrobacterial genes in the plant host, e.g. Arabidopsis thaliana. These genes are transferred by the T-DNA of the plant pathogen Agrobacterium tumefaciens and include the oncogenes IaaH, IaaM and Ipt, which, according to the tumor-inducing principle, are essential for crown gall development. Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). The oncogenes are involved in auxin and cytokinin production. The intergenic regions (IGRs) between the coding sequences (CDS) of the three oncogenes function as promoters in plant cells, but gene IaaM is constitutively expressed and no transcription factor further activates its promoter. With increasing auxin levels triggered by ubiquitous expression of IaaH and IaaM, ARF5 is activated and interacts with WRKY40 to potentiate Ipt expression and balance cytokinin and auxin levels for further cell proliferation. Transcriptional regulation of the Agrobacterium tumefaciens genes IaaH, IaaM and Ipt in the host plant, detailed overview
Agrobacterium tumefaciens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of auxin
Agrobacterium tumefaciens
physiological function
Crown gall development requires the expression of agrobacterial genes in the plant host, e.g. Arabidopsis thaliana. These genes are transferred by the T-DNA of the plant pathogen Agrobacterium tumefaciens and include the oncogenes IaaH, IaaM and Ipt, which, according to the tumor-inducing principle, are essential for crown gall development. Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). The oncogenes are involved in auxin and cytokinin production. The intergenic regions (IGRs) between the coding sequences (CDS) of the three oncogenes function as promoters in plant cells, but gene IaaM is constitutively expressed and no transcription factor further activates its promoter. With increasing auxin levels triggered by ubiquitous expression of IaaH and IaaM, ARF5 is activated and interacts with WRKY40 to potentiate Ipt expression and balance cytokinin and auxin levels for further cell proliferation. Transcriptional regulation of the Agrobacterium tumefaciens genes IaaH, IaaM and Ipt in the host plant, detailed overview
Agrobacterium tumefaciens
Expression (protein specific)
Organism
Commentary
Expression
Agrobacterium tumefaciens
gene IaaM is constitutively expressed and no transcription factor further activates its promoter
additional information
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746273
Zhang
Regulation of oncogene expres ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
PLoS Pathog.
11
1-27
2015
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745813
Yin
Characterization of a tryptop ...
Puccinia graminis f. sp. tritici, Puccinia graminis f. sp. tritici CRL 75-36-700-3
Mol. Plant Microbe Interact.
27
227-235
2014
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10
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2
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746257
Mujahid
Aniline is an inducer, and no ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
PLoS ONE
9
e87503
2014
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727017
Gaweska
Structure of the flavoprotein ...
Pseudomonas savastanoi
Biochemistry
52
2620-2626
2013
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726752
Mujahid
Production of indole-3-acetic ...
Rubrivivax benzoatilyticus
Appl. Microbiol. Biotechnol.
89
1001-1008
2011
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672116
Ralph
Mechanistic studies of the fla ...
Pseudomonas savastanoi
Biochemistry
45
15844-15852
2006
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654682
Sobrado
Analysis of the role of the ac ...
Pseudomonas savastanoi
Biochemistry
42
13826-13832
2003
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2
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6
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654683
Sobrado
Identification of Tyr413 as an ...
Pseudomonas savastanoi
Biochemistry
42
13833-13838
2003
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6
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439346
Sobrado
Analysis of the roles of amino ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
402
24-30
2002
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3
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439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
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439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
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439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
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16
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16
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439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
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439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
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439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
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439342
Comai
Involvement of plasmid deoxyri ...
Pseudomonas savastanoi
J. Bacteriol.
143
950-957
1980
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439341
Kosuge
Microbial synthesis and degrad ...
Pseudomonas savastanoi
J. Biol. Chem.
241
3738-3744
1966
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