Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 0.1 mM | Gaussia princeps |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Gaussia princeps |
Protein Variants | Comment | Organism |
---|---|---|
pCold-hGL | two repeat sequences, two catalytic domains | Gaussia princeps |
pCold-hGL-27/97 | one repeat sequence, with only one catalytic domain | Gaussia princeps |
pCold-hGL-98/168 | one repeat sequence, with only one catalytic domain | Gaussia princeps |
pCold-hSGL | with a signal peptide sequence for secretion | Gaussia princeps |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.01% | Gaussia princeps | |
CuCl2 | 1 microM, inhibition of 95% | Gaussia princeps | |
dithiothreitol | 1 mM | Gaussia princeps |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | insoluble fraction in Escherichia coli cells | Gaussia princeps | - |
- |
soluble | fraction in Escherichia coli cells | Gaussia princeps | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Br- | stimulates luminescence activity | Gaussia princeps | |
Cl- | stimulates luminescence activity | Gaussia princeps | |
Cu2+ | strong inhibition of luminescence activity | Gaussia princeps | |
I- | stimulates luminescence activity | Gaussia princeps |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10350 | - |
MALDI-TOF-MS analyses, hGL-98/168 | Gaussia princeps |
10740 | - |
MALDI-TOF-MS analyses, hGL-27/97 | Gaussia princeps |
20020 | - |
MALDI-TOF-MS analyses, hGL | Gaussia princeps |
25000 | - |
SDS-PAGE, pCold-hGL | Gaussia princeps |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaussia princeps | - |
- |
- |
Purification (Comment) | Organism |
---|---|
souble and insoluble fraction by nickel-chelate column | Gaussia princeps |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1170000000 relative light units/mg, hGL-27/97, soluble fraction | Gaussia princeps |
additional information | - |
14220000000 relative light units/mg, hGL, insoluble fraction | Gaussia princeps |
additional information | - |
20000000 relative light units/mg, hGL-98/168, insoluble fraction | Gaussia princeps |
additional information | - |
3120000000 relative light units/mg, hGL-27/97, insoluble fraction | Gaussia princeps |
additional information | - |
51230000000 relative light units/mg, hGL, soluble fraction | Gaussia princeps |
additional information | - |
590000000 relative light units/mg, hGL-98/168, soluble fraction | Gaussia princeps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
coelenterazine + O2 | 0.5 microg, assay at pH 7.6, high substrate specificity for coelenterazine | Gaussia princeps | coelenteramide + CO2 + hnu | - |
? | |
cp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
e-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
f-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
fcp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
h-coelenterazine + O2 | assay at pH 7.6m, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
hcp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
MeO-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
n-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gaussia luciferase | - |
Gaussia princeps |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Gaussia princeps |