Literature summary for 1.13.12.5 extracted from

Cissell, K.A.; Rahimi, Y.; Shrestha, S.; Deo, S.K.
Reassembly of a bioluminescent protein renilla luciferase directed through DNA hybridization (2009), Bioconjug. Chem., 20, 15-19.

Search for more literature for 1.13.12.5

Activating Compound

Activating Compound	Comment	Organism	Structure
(1,8-bis-maleimidotriethylene glycol)	effective conjugation of the N-terminal and C-terminal fragments of Rluc, results in enzymatic activity	Renilla sp.
additional information	hybridization of two oligonucleotide probes with fused Rluc fragments resulted in reassembly of the fragment, generating active Rluc	Renilla sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain ER 2566	Renilla sp.

Protein Variants

Protein Variants	Comment	Organism
G229stopstop	Rluc N-terminal fragment	Renilla sp.
RL8	mutant of Rluc	Renilla sp.
stop230	Rluc C-terminal fragment	Renilla sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	free oligonucleotide probe that competes to bind to the hybridizied probe-Rluc fragment complex inhibits the reassembly of Rluc, concentration-dependent; no activity of N-terminal or C-terminal Rluc fragments	Renilla sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
16000	-	value about, SDS-PAGE, C-terminal fragment	Renilla sp.
30000	-	value about, SDS-PAGE, N-terminal fragment	Renilla sp.

Organism

Organism	UniProt	Comment	Textmining
Renilla sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Hi-Trap column	Renilla sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coelenterazine + O2	-	Renilla sp.	coelenteramide + CO2 + hnu	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Renilla sp.

Synonyms

Synonyms	Comment	Organism
Renilla luciferase	-	Renilla sp.
RLuc	-	Renilla sp.

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Release 2023.1 (January 2023)