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Literature summary for 1.13.12.5 extracted from
- Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii (2008), Mol. Genet. Genomics, 280, 153-162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Chlamydomonas reinhardtii | Renilla sp. |
| expression in Chlamydomonas reinhardtii | Gaussia princeps |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gaussia princeps | Q9BLZ2 | - |
- |
| Renilla sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coelenterazine + O2 | 0.1 mM | Gaussia princeps | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gaussia-luciferase | - |
Gaussia princeps |
| Renilla-luciferase | - |
Renilla sp. |
| RLuc | - |
Renilla sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | at 50°C after 30 min incubation time only 20% residual activity, at 40°C after 1 h incubation time complete loss of activity | Gaussia princeps |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Gaussia princeps |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | below 6.5 and above 8.5 less than 20% residual activity | Gaussia princeps |
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Release 2023.1 (January 2023)
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