Cloned (Comment) | Organism |
---|---|
catalytic domain (19 kDa) is expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins | Oplophorus gracilirostris |
expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins | Renilla reniformis |
expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins | Gaussia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
catalytic domain | Oplophorus gracilirostris |
37600 | - |
SDS-PAGE | Renilla reniformis |
37700 | - |
calculated from sequence | Renilla reniformis |
38040 | - |
calculated from sequence, 4red-shift luciferase | Renilla reniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaussia | - |
- |
- |
Oplophorus gracilirostris | - |
- |
- |
Renilla reniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-chelate column | Renilla reniformis |
using Ni-chelate column | Oplophorus gracilirostris |
using Ni-chelate column | Gaussia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3iso-coelenterazine + O2 | relative activity to native coelenterazine: 11.6% | Renilla reniformis | ? | - |
? | |
3iso-coelenterazine + O2 | relative activity to native coelenterazine: 14.3% | Gaussia | ? | - |
? | |
3iso-coelenterazine + O2 | relative activity to native coelenterazine: 34.9% | Renilla reniformis | ? | - |
? | |
3iso-coelenterazine + O2 | relative activity to native coelenterazine: 78.2% | Oplophorus gracilirostris | ? | - |
? | |
3me-coelenterazine + O2 | relative activity to native coelenterazine: 11.8% | Renilla reniformis | ? | - |
? | |
3me-coelenterazine + O2 | relative activity to native coelenterazine: 2.6% | Gaussia | ? | - |
? | |
3me-coelenterazine + O2 | relative activity to native coelenterazine: 45.5% | Renilla reniformis | ? | - |
? | |
3me-coelenterazine + O2 | relative activity to native coelenterazine: 80% | Oplophorus gracilirostris | ? | - |
? | |
3meo-coelenterazine + O2 | relative activity to native coelenterazine: 189% | Oplophorus gracilirostris | ? | - |
? | |
3meo-coelenterazine + O2 | relative activity to native coelenterazine: 3.8% | Gaussia | ? | - |
? | |
3meo-coelenterazine + O2 | relative activity to native coelenterazine: 5.5% | Renilla reniformis | ? | - |
? | |
3meo-coelenterazine + O2 | relative activity to native coelenterazine: 82.3% | Renilla reniformis | ? | - |
? | |
alphameh-coelenterazine + O2 | relative activity to native coelenterazine: 0.02% | Renilla reniformis | ? | - |
? | |
alphameh-coelenterazine + O2 | relative activity to native coelenterazine: 0.02% | Gaussia | ? | - |
? | |
alphameh-coelenterazine + O2 | relative activity to native coelenterazine: 15.7% | Oplophorus gracilirostris | ? | - |
? | |
alphameh-coelenterazine + O2 | relative activity to native coelenterazine: 24.7% | Renilla reniformis | ? | - |
? | |
cf3-coelenterazine + O2 | relative activity to native coelenterazine: 0.8% | Gaussia | ? | - |
? | |
cf3-coelenterazine + O2 | relative activity to native coelenterazine: 16.8% | Renilla reniformis | ? | - |
? | |
cf3-coelenterazine + O2 | relative activity to native coelenterazine: 49.5% | Oplophorus gracilirostris | ? | - |
? | |
cf3-coelenterazine + O2 | relative activity to native coelenterazine: 5% | Renilla reniformis | ? | - |
? | |
coelenterazine + O2 | relative activity: 100% | Renilla reniformis | ? | - |
? | |
coelenterazine + O2 | relative activity: 100% | Oplophorus gracilirostris | ? | - |
? | |
coelenterazine + O2 | relative activity: 100% | Gaussia | ? | - |
? | |
et-coelenterazine + O2 | relative activity to native coelenterazine: 0.9% | Gaussia | ? | - |
? | |
et-coelenterazine + O2 | relative activity to native coelenterazine: 1.2% | Renilla reniformis | ? | - |
? | |
et-coelenterazine + O2 | relative activity to native coelenterazine: 21.1% | Oplophorus gracilirostris | ? | - |
? | |
et-coelenterazine + O2 | relative activity to native coelenterazine: 78.3% | Renilla reniformis | ? | - |
? | |
h-coelenterazine + O2 | relative activity to native coelenterazine: 12.4% | Renilla reniformis | ? | - |
? | |
h-coelenterazine + O2 | relative activity to native coelenterazine: 5.8% | Gaussia | ? | - |
? | |
h-coelenterazine + O2 | relative activity to native coelenterazine: 54.1% | Renilla reniformis | ? | - |
? | |
h-coelenterazine + O2 | relative activity to native coelenterazine: 68.4% | Oplophorus gracilirostris | ? | - |
? | |
i-coelenterazine + O2 | relative activity to native coelenterazine: 0.3% | Renilla reniformis | ? | - |
? | |
i-coelenterazine + O2 | relative activity to native coelenterazine: 0.9% | Gaussia | ? | - |
? | |
i-coelenterazine + O2 | relative activity to native coelenterazine: 32.3% | Oplophorus gracilirostris | ? | - |
? | |
i-coelenterazine + O2 | relative activity to native coelenterazine: 5.1% | Renilla reniformis | ? | - |
? | |
me-coelenterazine + O2 | relative activity to native coelenterazine: 4.5% | Gaussia | ? | - |
? | |
me-coelenterazine + O2 | relative activity to native coelenterazine: 46.6% | Oplophorus gracilirostris | ? | - |
? | |
me-coelenterazine + O2 | relative activity to native coelenterazine: 5.2% | Renilla reniformis | ? | - |
? | |
me-coelenterazine + O2 | relative activity to native coelenterazine: 73.6% | Renilla reniformis | ? | - |
? | |
meo-coelenterazine + O2 | relative activity to native coelenterazine: 1.2% | Renilla reniformis | ? | - |
? | |
meo-coelenterazine + O2 | relative activity to native coelenterazine: 13.6% | Gaussia | ? | - |
? | |
meo-coelenterazine + O2 | relative activity to native coelenterazine: 18.2% | Renilla reniformis | ? | - |
? | |
meo-coelenterazine + O2 | relative activity to native coelenterazine: 68.1% | Oplophorus gracilirostris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
19kOLase | - |
Oplophorus gracilirostris |
GLase | - |
Gaussia |
luciferase | - |
Renilla reniformis |
luciferase | - |
Oplophorus gracilirostris |
luciferase | - |
Gaussia |
Oplophorus luciferase | - |
Oplophorus gracilirostris |
Renilla luciferase | - |
Renilla reniformis |
Renilla luciferase-547 | red shift variant | Renilla reniformis |
RLase | - |
Renilla reniformis |
RLase-547 | red shift variant | Renilla reniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Gaussia |
7.6 | - |
assay at | Renilla reniformis |
7.6 | - |
assay at | Oplophorus gracilirostris |