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Literature summary for 1.13.12.5 extracted from
- Renilla luciferase-LC3 based reporter assay for measuring autophagic flux (2017), Methods Enzymol., 588, 1-13 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | establishment and evaluation of an indirect autophagy flux assay based on monitoring the degradation of an autophagosome-associated fusion protein Rluc-LC3 by luminescence detection. The Rluc-LC3 assay is useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells | Renilla reniformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the enzyme is fused to wild-type LC3 protein and LC3 mutant G120A. LC3-II turnover is used as a marker of autophagic flux. The Rluc-LC3 fusion protein is used for the indirect autophagy flux assay based on monitoring the degradation of an autophagosome-associated fusion protein Rluc-LC3 by luminescence detection. The Rluc-LC3 assay is useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells. Design of the RLUC-LC3wt and RLUC-LC3G120A fusion proteins, recombinantly expressed in MCF-7 cells. Method evaluation and optimization, overview | Renilla reniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Renilla reniformis | P27652 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | establishment and evaluation of an indirect autophagy flux assay based on monitoring the degradation of an autophagosome-associated fusion protein Rluc-LC3 by luminescence detection. The Rluc-LC3 assay is useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells. LC3 is a subfamily in the Atg8 family of ubiquitin-like proteins and is the only protein marker described to reliably bind the autophagosomal membrane and the phagophore Method evaluation and optimization, overview | Renilla reniformis | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Renilla luciferase | - |
Renilla reniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Renilla reniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Renilla reniformis |
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