Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | substitution of NaCl by KCl does not significantly alter the dependence of light intensity on salt concentrations | Gaussia princeps | |
NaCl | maximum activity at 1.0-1.5 M, a high NaCl concentration enhances luciferase stability to thermal denaturation | Gaussia princeps |
Cloned (Comment) | Organism |
---|---|
expression in insect cells using a baculovirus expression system | Gaussia princeps |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00214 | - |
coelenterazine | K0.5 value, Hill coefficient 1.8, pH 7.5, temperature not specified in the publication | Gaussia princeps |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaussia princeps | Q9BLZ2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | enzyme contains five intramolecular S-S bonds per molecule | Gaussia princeps |
Purification (Comment) | Organism |
---|---|
recombinant secreted enzyme | Gaussia princeps |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, stable for months | Gaussia princeps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Gaussia princeps | ? + CO2 + hv | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 20 | - |
Gaussia princeps |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
1 | - |
30% residual activity | Gaussia princeps |
37 | - |
20% residual activity | Gaussia princeps |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | - |
melting temperature | Gaussia princeps |
77 | - |
melting temperature, presence of 1 M NaCl | Gaussia princeps |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Gaussia princeps |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | - |
60% residual activity | Gaussia princeps |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme shows positive cooperativity with coelenterazine, rather due to the kinetic cooperativity conditioned by conformational changes in response to substrate binding than to the presence of two catalytic sites | Gaussia princeps |