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Literature summary for 1.13.12.6 extracted from
- Activity and inhibition of Cypridina luciferase quantitative measurement, analysis of inhibition by urea, and some effects of sodium and potassium ions (2015), Biolumin. Progr., 2015, 115-136 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | at 5.0 M urea, the half-time of the enzyme is 45 min, at 6.0 M urea 8 min, and at 7.5 M urea, the enzyme is inactivated within 1 min. r´Reversible, non-competitive inhibition of the luciferase activity occurs at concentrations up to 1.5 M | Cypridina sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Cypridina sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates the enzyme by about 50% at higher pH values | Cypridina sp. | |
| Na+ | activates the enzyme by about 50% at lower pH values | Cypridina sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cypridina luciferin + O2 | Cypridina sp. | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cypridina sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cypridina luciferin + O2 | - |
Cypridina sp. | oxidized Cypridina luciferin + CO2 + hv | - |
? | |
| additional information | evaluation of a method of measuring activity of Cypridina luciferase under conditions which minimize complicating with a relatively simple photoelectric light integrator, detailed overview | Cypridina sp. | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cypridina luciferase | - |
Cypridina sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Cypridina sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Cypridina sp. |
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