Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cypndina gamma-globulin | Cypridina luciferase is completely inhibited by a rabbit antibody to Cypridina luciferase | Vargula hilgendorfii | |
additional information | Apogon luciferase is not inhibited by a rabbit antibody to Cypridina luciferase | Apogon ellioti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Vargula hilgendorfii | |
additional information | - |
additional information | Michaelis-Menten kinetics | Apogon ellioti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cypridina luciferin + O2 | Vargula hilgendorfii | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? | |
Cypridina luciferin + O2 | Apogon ellioti | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apogon ellioti | - |
- |
- |
Vargula hilgendorfii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration | Vargula hilgendorfii |
native enzyme from photogenic organs by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration | Apogon ellioti |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
photogenic organ | - |
Vargula hilgendorfii | - |
photogenic organ | - |
Apogon ellioti | - |
Storage Stability | Organism |
---|---|
Cypridina luciferase occasionally loses almost all of its activity when chromatographed through the 90-cm Sephadex column, irrespective of whether the column is newly poured or has been used for a period. The loss also occurs with both newly dissolved and old solutions of Cypridina luciferase | Vargula hilgendorfii |
purified native Apogon luciferase gradually loses activity on standing in the cold over a period of weeks | Apogon ellioti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cypridina luciferin + O2 | - |
Vargula hilgendorfii | oxidized Cypridina luciferin + CO2 + hv | - |
? | |
Cypridina luciferin + O2 | - |
Apogon ellioti | oxidized Cypridina luciferin + CO2 + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Apogon luciferase | - |
Apogon ellioti |
Apogon luciferase 1 | - |
Apogon ellioti |
Cypridina luciferase | - |
Vargula hilgendorfii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0147 | - |
Cypridina luciferin | pH 6.8, 26°C | Vargula hilgendorfii | |
0.0152 | - |
Cypridina luciferin | pH 6.8, 26°C | Apogon ellioti |