Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.7 extracted from

  • Nakamura, M.; Maki, S.; Amano, Y.; Ohkita, Y.; Niwa, K.; Hirano, T.; Ohmiya, Y.; Niwa, H.
    Firefly luciferase exhibits bimodal action depending on the luciferin chirality (2005), Biochem. Biophys. Res. Commun., 331, 471-475.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-luciferin inhibits CoA-ligase activity with L-luciferin, IC50: 0.135 mM against 0.1 mM L-luciferin Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0982
-
L-luciferin
-
Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroluciferin + CoA
-
Photinus pyralis dehydroluciferyl-CoA
-
?
L-luciferin + CoA + ATP L-luciferin is converted to luciferyl adenylate, and the adenyl group of luciferyl adenylate is then substituted to CoA-SH to give luciferyl-CoA. Even in presence of CoA-SH, D-luciferin is used for the light production reaction, but is not converted into luciferyl-CoA Photinus pyralis luciferyl-CoA + AMP + diphosphate
-
?
luciferin + O2 + ATP
-
Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.135
-
inhibits CoA-ligase activity with L-luciferin, IC50: 0.135 mM against 0.1 mM L-luciferin Photinus pyralis D-luciferin