Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.7 extracted from

  • Oba, Y.; Tanaka, K.; Inouye, S.
    Catalytic properties of domain-exchanged chimeric proteins between firefly luciferase and Drosophila fatty acyl-CoA synthetase CG6178 (2006), Biosci. Biotechnol. Biochem., 70, 2739-2744.
    View publication on PubMed
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Photinus pyralis

Protein Variants

Protein Variants Comment Organism
additional information a chimeric protein with the N-terminal domain of firefly luciferase and the C-terminal domain of the firefly luciferase homolog from Drosophila melanogaster CG6178 shows approximately 4% of the activity of wild type firefly luciferase Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65500
-
 calculated from amino acid sequence Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis P08659
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelate affinity chromatography Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + O2 + ATP
-
 Photinus pyralis oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
fatty acid + ATP + CoA
-
 Photinus pyralis fatty acyl-CoA + AMP
-
 ?
luciferyl-O-adenosine monophosphate + CoA
-
 Photinus pyralis luciferyl-CoA + AMP
-
 ?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
 Photinus pyralis
Photinus pyralis luciferase
-
 Photinus pyralis
PpLase
-
 Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Photinus pyralis

pI Value

Organism Comment pI Value Maximum pI Value
Photinus pyralis calculated with ExPASy Compute pI/MW tool
-
 6.2