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Literature summary for 1.13.12.7 extracted from

  • Viviani, V.R.; Silva Neto, A.J.; Arnoldi, F.G.; Barbosa, J.A.; Ohmiya, Y.
    The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: a solvent gate for the active site of pH-sensitive luciferases (2007), Photochem. Photobiol., 84, 138-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Phrixothrix vivianii
expressed in Escherichia coli XL1-Blue cells Cratomorphus distinctus

Protein Variants

Protein Variants Comment Organism
F227Y the mutation causes dramatic redshift and temporal shift Cratomorphus distinctus
F227Y the mutation results in a unique time-dependent shift from red to yellow-green Macrolampis sp2
F260L the mutation results in a much less pH-sensitive enzyme displaying only a 10 nm redshift and broadening of the spectra at pH 6 Cratomorphus distinctus
G228A the mutation results in a large redshift Phrixothrix vivianii
G228A the mutation results in a large redshift Macrolampis sp2
I226A the mutation causes dramatic redshift and temporal shift Cratomorphus distinctus
L260F mutation results in a 20 nm redshift without affecting pH-insensitivity Pyrearinus termitilluminans
V227A mutant displays a small redshift Pyrearinus termitilluminans
V227F mutation results in a 60 nm redshift Pyrearinus termitilluminans
V227I mutant displays a small redshift Pyrearinus termitilluminans
V227S mutant displays a small redshift Pyrearinus termitilluminans
Y227A mutant shows enzymatic behaviour similar to the wild type enzyme Phrixothrix hirtus
Y227A the mutation causes dramatic red- and time-dependent shifts Phrixothrix vivianii
Y227A the substitution results in a weak and unstable red mutant Macrolampis sp2

Organism

Organism UniProt Comment Textmining
Cratomorphus distinctus Q5USC8
-
-
Macrolampis sp2
-
-
-
Phrixothrix hirtus Q9U4U7
-
-
Phrixothrix vivianii Q9U4U8
-
-
Pyrearinus termitilluminans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
agarose-Ni2+ column chromatography Phrixothrix vivianii
agarose-Ni2+ column chromatography Pyrearinus termitilluminans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + O2 + ATP
-
Cratomorphus distinctus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?
D-luciferin + O2 + ATP
-
Phrixothrix vivianii oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?
D-luciferin + O2 + ATP
-
Macrolampis sp2 oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?
D-luciferin + O2 + ATP
-
Pyrearinus termitilluminans oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?
D-luciferin + O2 + ATP
-
Phrixothrix hirtus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
beetle luciferase
-
Cratomorphus distinctus
beetle luciferase
-
Phrixothrix vivianii
beetle luciferase
-
Macrolampis sp2
beetle luciferase
-
Pyrearinus termitilluminans
beetle luciferase
-
Phrixothrix hirtus
firefly luciferase
-
Cratomorphus distinctus
firefly luciferase
-
Phrixothrix vivianii
firefly luciferase
-
Macrolampis sp2
firefly luciferase
-
Pyrearinus termitilluminans
firefly luciferase
-
Phrixothrix hirtus