Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) as His-tag fusion protein | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-phenylalanine | pH 8.0, 25°C | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + O2 | Pseudomonas sp. | - |
2-phenylacetamide + CO2 + H2O | - |
? | |
L-phenylalanine + O2 | Pseudomonas sp. P-501 | - |
2-phenylacetamide + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q5W9R9 | - |
- |
Pseudomonas sp. P-501 | Q5W9R9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + O2 | - |
Pseudomonas sp. | 2-phenylacetamide + CO2 + H2O | - |
? | |
L-phenylalanine + O2 | - |
Pseudomonas sp. P-501 | 2-phenylacetamide + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
103 | - |
L-phenylalanine | pH 8.0, 25°C | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Pseudomonas sp. |