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Literature summary for 1.13.99.1 extracted from
- Transcriptional and translational modulation of myo-inositol oxygenase (Miox) by fatty acids implications in renal tubular injury induced in obesity and diabetes (2016), J. Biol. Chem., 291, 1348-1367 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| palmitate | concomitant treatment with palmitate/bovine serum albumin and activators of PKA (forskolin), PDK/PI3K (insulin), and PKC (TPA) increase Miox activity. Concomitant treatment with respective inhibitors, i.e. H89 (PKA), wortmannin (PI3K), and calphostin (PKC), reduces the activity below the levels induced by palmitate/bovine serum albumin alone, confirming that fatty acid-induced activity is phosphorylation-dependent | Sus scrofa |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MIOX, quantitative real-time PCR enzyme expression analysis | Mus musculus |
| gene MIOX, quantitative real-time PCR enzyme expression analysis | Rattus norvegicus |
| gene MIOX, quantitative real-time PCR enzyme expression analysis | Homo sapiens |
| gene MIOX, quantitative real-time PCR enzyme expression analysis | Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Sus scrofa | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | Mus musculus | - |
D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | Rattus norvegicus | - |
D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | Homo sapiens | - |
D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | Sus scrofa | - |
D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | Mus musculus CD1 | - |
D-glucuronate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UGB7 | - |
- |
| Mus musculus | Q9QXN5 | - |
- |
| Mus musculus CD1 | Q9QXN5 | - |
- |
| Rattus norvegicus | Q9QXN4 | - |
- |
| Sus scrofa | Q8WN98 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HK-2 cell | - |
Homo sapiens | - |
| kidney | - |
Mus musculus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Homo sapiens | - |
| kidney | - |
Sus scrofa | - |
| LLC-PK1 cell | - |
Sus scrofa | - |
| additional information | immunohistochemic analysis | Mus musculus | - |
| additional information | immunohistochemic analysis | Rattus norvegicus | - |
| additional information | immunohistochemic analysis | Homo sapiens | - |
| additional information | immunohistochemic analysis | Sus scrofa | - |
| renal tubule | MIOX is a tubular-specific enzyme | Mus musculus | - |
| renal tubule | MIOX is a tubular-specific enzyme | Rattus norvegicus | - |
| renal tubule | MIOX is a tubular-specific enzyme | Homo sapiens | - |
| renal tubule | MIOX is a tubular-specific enzyme | Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | - |
Mus musculus | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Rattus norvegicus | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Homo sapiens | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Sus scrofa | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Mus musculus CD1 | D-glucuronate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MIOX | - |
Mus musculus |
| MIOX | - |
Rattus norvegicus |
| MIOX | - |
Homo sapiens |
| MIOX | - |
Sus scrofa |
| Myo-inositol oxygenase | - |
Mus musculus |
| Myo-inositol oxygenase | - |
Rattus norvegicus |
| Myo-inositol oxygenase | - |
Homo sapiens |
| Myo-inositol oxygenase | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mus musculus |
| 8 | - |
assay at | Rattus norvegicus |
| 8 | - |
assay at | Homo sapiens |
| 8 | - |
assay at | Sus scrofa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | transcriptional and translational modulation of myo-inositol oxygenase (Miox) by fatty acids, overview | additional information |
| Rattus norvegicus | transcriptional and translational modulation of myo-inositol oxygenase (Miox) by fatty acids, overview | additional information |
| Homo sapiens | transcriptional and translational modulation of myo-inositol oxygenase (Miox) by fatty acids, overview | additional information |
| Sus scrofa | transcriptional and translational modulation of myo-inositol oxygenase (Miox) by fatty acids, overview | additional information |
| Mus musculus | administration of high-fat diet to CD1 mice over a period of 2-6 weeks induces a tremendous increase in the expression of Miox, exclusively confined to the tubular compartment of the kidney cortex. Besides the increased expression in superficial cortical tubules, it also extends into the deeper cortex. Upregulation of Miox is accompanied by upregulation of mSrebp1 in kidney cells. Rapamycin reverses palmitate/bovine serum albumin-induced Miox, Srebp1, and p53 expression and apoptosis in renal tubular cells | up |
| Rattus norvegicus | high-fat diet administration over a period of 6 weeks results in a marked time-dependent up-regulation of Miox | up |
| Sus scrofa | palmitate-conjugated bovine serum albumin causes a dose-dependent increase in Miox expression and activity in LLCPK-1 cells. Concomitant with Miox upregulation, a dose-dependent increased Bax protein expression is observed following palmitate/BSA treatment of LLCPK-1 cells. Concomitant treatment with palmitate/bovine serum albumin and activators of PKA (forskolin), PDK/PI3K (insulin), and PKC (TPA) further increase Miox activity | up |
| Homo sapiens | treatment of HK-2 cells with palmitate/bovine serum albumin for 24 h induces an increased Miox expression with a concomitant decrease in the membrane-bound precursor form of pre-Srebp1 in the cytoplasmic fraction. No change in the expression of beta-actin or laminB1 is observed. Miox is transcriptionally upregulated by high glucose ambience. A dose-dependent increase in the expression of Miox is observed following insulin treatment. At the same time, a dose-dependent increase in the mSrebp1 is observed. Rapamycin reverses palmitate/bovine serum albumin-induced Miox, Srebp1, and p53 expression and apoptosis in renal tubular cells | up |
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