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Literature summary for 1.13.99.3 extracted from
- Isolation and reconstitution of two electron transfer components of tryptophan side chain oxidase (1979), J. Biol. Chem., 254, 11794-11797.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Pseudomonas fluorescens |  |
| indole-3-methanol | inhibits oxidation of skatole | Pseudomonas fluorescens | |
| KCN | - |
Pseudomonas fluorescens | |
| skatole | inhibits oxidation of indole-3-methanol | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
indole-3-methanol | - |
Pseudomonas fluorescens | |
| 0.67 | - |
ferricyanide | - |
Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | dehydrogenase component: 0.67 atoms of iron per mol of component | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
| 72000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
ATCC 29574 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dehydrogenase component and oxidase component | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.2 | - |
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-indolemethanol + ferricyanide | - |
Pseudomonas fluorescens | ? | - |
r | |
| 3-indolemethanol + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
| additional information | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes (e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol) but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
| dimer | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol, but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | hemoprotein | Pseudomonas fluorescens | |
| heme | dehydrogenase component: 0.73 mol protoheme IX per mol of component | Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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