Literature summary for 1.14.11.1 extracted from

Rydzik, A.M.; Brem, J.; Struwe, W.B.; Kochan, G.T.; Benesch, J.L.; Schofield, C.J.
Ejection of structural zinc leads to inhibition of gamma-butyrobetaine hydroxylase (2014), Bioorg. Med. Chem. Lett., 24, 4954-4957 .

Search for more literature for 1.14.11.1

Application

Application	Comment	Organism
medicine	inhibition of BBOX is reported to aid in recovery after acute stroke	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
benzeneselenenic acid	-	Homo sapiens
benzeneselenenyl bromide	-	Homo sapiens
benzeneselenenyl chloride	-	Homo sapiens
benzeneseleninic acid	-	Homo sapiens
diphenyldiselane	-	Homo sapiens
ebselen	a relatively potent BBOX inhibitor and a Zn(II) ejector	Homo sapiens
N-oxalylglycine	a 2-oxoglutarate mimic	Homo sapiens
N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine	i.e. FG-2216, BIQ, or IOX3, an isoquinoline derivative, that inhibits BBOX and other 2-oxoglutarate dependent oxygenases through chelating active site Fe(II) and interacting with specific active site residues. Time course of Zn(II) ejection from BBOX by the inhibitors	Homo sapiens
thiram	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	dependent on, required for catalysis, bound to the active site	Homo sapiens
Zn2+	dependent on, ejection of structural zinc, e.g. by Selenium and sulfur based compounds, leads to inhibition of the enzyme, an undisrupted N-terminal zinc binding domain of BBOX is required for catalysis	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-trimethylammoniobutanoate + 2-oxoglutarate + O2	Homo sapiens	-	3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O75936	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-trimethylammoniobutanoate + 2-oxoglutarate + O2	-	Homo sapiens	3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2	-	?

Subunits

Subunits	Comment	Organism
homodimer	BBOX is a homodimer comprising of catalytic domain where Fe(II) and substrates bind and zinc binding domain	Homo sapiens

Synonyms

Synonyms	Comment	Organism
BBOX	-	Homo sapiens
gamma-butyrobetaine hydroxylase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00083	-	pH 7.0, 22°C, 20 min	Homo sapiens	ebselen
0.0029	-	pH 7.0, 22°C, 20 min	Homo sapiens	benzeneselenenyl chloride
0.003	-	pH 7.0, 22°C, 20 min	Homo sapiens	benzeneselenenyl bromide
0.0047	-	pH 7.0, 22°C, 20 min	Homo sapiens	benzeneseleninic acid
0.0079	-	pH 7.0, 22°C, 20 min	Homo sapiens	thiram
0.019	-	pH 7.0, 22°C, 20 min	Homo sapiens	benzeneselenenic acid
0.032	-	pH 7.0, 22°C, 20 min	Homo sapiens	N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine
0.042	-	pH 7.0, 22°C, 20 min	Homo sapiens	diphenyldiselane

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the 2-oxoglutarate/Fe(II) dependent oxygenase family	Homo sapiens
metabolism	the enzyme catalyses the second, essential step of carnitine biosynthesis	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)