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Literature summary for 1.14.11.11 extracted from
- Functional identification of hyoscyamine 6beta-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli (2011), Biotechnol. Lett., 33, 1361-1365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3) | Anisodus acutangulus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
| 64000 | - |
x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-hyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
(6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anisodus acutangulus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His- or GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel or glutathione affinity chromatography, respectively | Anisodus acutangulus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-hyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| additional information | the enzyme also catalyzes the reaction of EC 1.14.11.14 | Anisodus acutangulus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Anisodus acutangulus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H6H | - |
Anisodus acutangulus |
| hyoscyamine 6beta-hydroxylase | - |
Anisodus acutangulus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | hyoscyamine 6beta-hydroxylase converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway | Anisodus acutangulus |
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Release 2023.1 (January 2023)
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