Cardillo, A.; Perassolo, M.; Sartuqui, M.; Rodriguez Talou, J.; Giulietti, A.
Production of tropane alkaloids by biotransformation using recombinant Escherichia coli whole cells (2017), Biochem. Eng. J., 125, 180-189 .
No PubMed abstract available
Application
Application |
Comment |
Organism |
drug development |
tropane alkaloids, such as hyoscyamine, 6-hydroxyhyoscyamine and scopolamine, are secondary metabolites that are traditionally applied in medicine due to their anticholinergic activity. An alternative strategy for the production of the most valuable alkaloids, 6beta-hydroxyhyoscyamine and scopolamine, using Escherichia coli harboring the hyoscyamine-6beta-hydroxylase enzyme as biocatalysts is developed |
Brugmansia x candida |
Cloned(Commentary)
Cloned (Comment) |
Organism |
the h6hcDNA is amplified from Brugmansia candida total RNA preparations, it is inserted in frame to the trx tag into the pET32a(+) vector. Escherichia coli Origami strains are used as host for the expression. Produce of high quantities of a soluble and functional enzyme |
Brugmansia x candida |
Organism
Organism |
UniProt |
Comment |
Textmining |
Brugmansia x candida |
B2CNC8 |
- |
- |