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Literature summary for 1.14.11.11 extracted from

  • Kluza, A.; Wojdyla, Z.; Mrugala, B.; Kurpiewska, K.; Porebski, P.J.; Niedzialkowska, E.; Minor, W.; Weiss, M.S.; Borowski, T.
    Regioselectivity of hyoscyamine 6beta-hydroxylase-catalysed hydroxylation as revealed by high-resolution structural information and QM/MM calculations (2020), Dalton Trans., 49, 4454-4469 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Datura metel

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour diffusion method Datura metel

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Datura metel

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2 Datura metel
-
(6S)-hydroxyhyoscyamine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Datura metel Q6EZB3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Datura metel

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2
-
Datura metel (6S)-hydroxyhyoscyamine + succinate + CO2
-
?
L-hyoscyamine + 2-oxoglutarate + O2 Glu116 interacts with the tertiary amine located on the hyoscyamine tropane moiety and Tyr326 forms CH-pi hydrogen bonds with the hyoscyamine phenyl ring Datura metel (6S)-hydroxyhyoscyamine + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
H6H
-
Datura metel
hyoscyamine 6beta-hydroxylase
-
Datura metel

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of scopolamine Datura metel