Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Datura metel |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour diffusion method | Datura metel |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Datura metel |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hyoscyamine + 2-oxoglutarate + O2 | Datura metel | - |
(6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura metel | Q6EZB3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Datura metel |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hyoscyamine + 2-oxoglutarate + O2 | - |
Datura metel | (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
L-hyoscyamine + 2-oxoglutarate + O2 | Glu116 interacts with the tertiary amine located on the hyoscyamine tropane moiety and Tyr326 forms CH-pi hydrogen bonds with the hyoscyamine phenyl ring | Datura metel | (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H6H | - |
Datura metel |
hyoscyamine 6beta-hydroxylase | - |
Datura metel |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of scopolamine | Datura metel |