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Literature summary for 1.14.11.15 extracted from

  • Kim, S.G.; Lee, A.K.; Yoon, H.K.; Park, C.M.
    A membrane-bound NAC transcription factor NTL8 regulates gibberellic acid-mediated salt signaling in Arabidopsis seed germination (2008), Plant J., 55, 77-88.
    View publication on PubMed
Search for more literature for 1.14.11.15

Cloned(Commentary)

Cloned (Comment) Organism
for the NTL8 complementation test, a sequence containing the NTL8 gene with its promoter is subcloned into pKGWFS7 Gateway vector, for histochemical staining an NTL8 promoter region is transcriptionally fused to the GUS-coding sequence, the fusion product transformed into Arabidopsis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information transgenic plants overexpressing an active NTL8 form and T-DNA insertional ntl8-1 mutant, under standard conditions, germinate with the same rate, under high salinity stress (150 mM NaCl) the germination of the NTL8 overexpressing mutant is considerably reduced while the NTL8-deficient mutant is less sensitive, independent of absiasic acid (ABA), NTL8-deficient mutant is not impaired by gibberellin biosynthetic inhibitor paclabutrazol (PAC), gibberellin downregulates NTL8 lowering its threshold for germination Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 150 mM NaCl represses the gibberellin 3-oxidase 1 gene in the wild-type and in the NTL8-deficient mutant (NTL8 acts downstream of the gibberellin biosynthesis), repression is present but less significant in aba3-1 mutant, deficient in the stress hormone abscisic acid (independent pathway dominates), expression of GA3ox1 is recovered after a longer incubation in NaCl and leads to germination Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gibberellin 20 + 2-oxoglutarate + O2 Arabidopsis thaliana
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 gibberellin 1 + succinate + CO2
-
 ?
additional information Arabidopsis thaliana gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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 variety Columbia (Col-0), transgenic 35S:8deltaC (overexpressing active NTL8) and ntl8-1 (lacking NTL8) mutants, aba3-1 (deficient in stress hormone abscisic acid - ABA) mutant
-

Purification (Commentary)

Purification (Comment) Organism
transgenic plants are ground in liquid nitrogen, total cellular extracts suspended in 1X SDS-PAGE sample loading buffer Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin 20 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana gibberellin 1 + succinate + CO2
-
 ?
additional information gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
GA3 oxidase 1
-
 Arabidopsis thaliana
GA3ox1
-
 Arabidopsis thaliana
gibberellin 3 oxidase 1
-
 Arabidopsis thaliana