KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
Fe2+ | 56 kDa protein | Bos taurus | |
0.0096 | - |
Fe2+ | 52 kDa protein | Bos taurus | |
0.021 | - |
2-oxoglutarate | 56 kDa protein | Bos taurus | |
0.022 | - |
2-oxoglutarate | 52 kDa protein | Bos taurus | |
0.034 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein | Bos taurus | |
0.035 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein | Bos taurus | |
0.067 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S, 52 kDa protein | Bos taurus | |
0.075 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S, 56 kDa protein | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE, protein with lower molecular weight | Bos taurus |
56000 | - |
SDS-PAGE, protein with higher molecular weight | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two enzymes, one 52000 Da, the other 56000 Da | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl pH 7.2, 1 mg/ml bovine serum albumin, at least 8 weeks stable | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
first epidermal growth factor-like domain-L-aspartate + 2-oxoglutarate + O2 | - |
Bos taurus | first epidermal growth factor-like domain-3-hydroxy-L-aspartate + succinate + CO2 | - |
r | |
peptide L-asparagine + 2-oxoglutarate + O2 | first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18 | Bos taurus | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? | |
peptide L-aspartate + 2-oxoglutarate + O2 | first epidermal growth factor-like domain of human protein S as substrate | Bos taurus | peptide 3-hydroxy-L-aspartate + succinate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S, 56 kDa protein | Bos taurus | |
0.0217 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein | Bos taurus | |
0.025 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S, 52 kDa protein | Bos taurus | |
0.0317 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein | Bos taurus |