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Literature summary for 1.14.11.16 extracted from

  • Pfeffer, I.; Brewitz, L.; Krojer, T.; Jensen, S.A.; Kochan, G.T.; Kershaw, N.J.; Hewitson, K.S.; McNeill, L.A.; Kramer, H.; Muenzel, M.; Hopkinson, R.J.; Oppermann, U.; Handford, P.A.; McDonough, M.A.; Schofield, C.J.
    Aspartate/asparagine-beta-hydroxylase crystal structures reveal an unexpected epidermal growth factor-like domain substrate disulfide pattern (2019), Nat. Commun., 10, 4910 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the N-terminally His6-tagged AspH315-758 is transformed into Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of N-terminally His6-tagged AspH562-758 are grown by the vapour diffusion method at 20°C in sitting drops, crystal structures of the enzyme, with and without substrate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane-anchored enzyme, whose C-terminal oxygenase and tetratricopeptide repeat domains present in the ER lumen Homo sapiens 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe(II)-binding employs only two Fe(II)-binding ligands (His679/His725) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-aspartate + 2-oxoglutarate + O2 Homo sapiens the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains peptide-3-hydroxy-L-aspartate + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q12797
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptide-L-aspartate + 2-oxoglutarate + O2
-
Homo sapiens peptide-3-hydroxy-L-aspartate + succinate + CO2
-
?
peptide-L-aspartate + 2-oxoglutarate + O2 the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains Homo sapiens peptide-3-hydroxy-L-aspartate + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
aspartate/asparagine-beta-hydroxylase
-
Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains Homo sapiens