Cloned (Comment) | Organism |
---|---|
the N-terminally His6-tagged AspH315-758 is transformed into Escherichia coli BL21 (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystals of N-terminally His6-tagged AspH562-758 are grown by the vapour diffusion method at 20°C in sitting drops, crystal structures of the enzyme, with and without substrate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | membrane-anchored enzyme, whose C-terminal oxygenase and tetratricopeptide repeat domains present in the ER lumen | Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe(II)-binding employs only two Fe(II)-binding ligands (His679/His725) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-aspartate + 2-oxoglutarate + O2 | Homo sapiens | the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains | peptide-3-hydroxy-L-aspartate + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q12797 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-aspartate + 2-oxoglutarate + O2 | - |
Homo sapiens | peptide-3-hydroxy-L-aspartate + succinate + CO2 | - |
? | |
peptide-L-aspartate + 2-oxoglutarate + O2 | the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains | Homo sapiens | peptide-3-hydroxy-L-aspartate + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate/asparagine-beta-hydroxylase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains | Homo sapiens |