Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ | Homo sapiens | |
GTP | at 0.1 mM Fe2+ 4 mM GTP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM GTP for 1.0 mM Fe2+ | Homo sapiens | |
imidazole | at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ | Homo sapiens | |
imidazole | stimulates at 1.0 mM Fe2+ | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D177A | no activity with phytanoyl-CoA | Homo sapiens |
H175A | no activity with phytanoyl-CoA | Homo sapiens |
H213A | insoluble mutant enzyme | Homo sapiens |
H264A | activity with phytanoyl-CoA is 7.5 of the wild-type activity | Homo sapiens |
Q176A | activity with phytanoyl-CoA is 16.3% of the wild-type activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ | Homo sapiens | |
GTP | at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ required. Maximal activity is 0.05-0.1 mM Fe2+ in absence of ATP | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14832 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | unprocessed proenzyme contains a N-terminal peroxisomal targeting sequence that is cleaved to give mature PAHX. Both forms are able to hydroxylate a range of CoA derivatives, but under the same assay conditions, the N-terminal Hexa-His-tagged unprocessed form is less active than the nontagged mature form | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PAHX | - |
Homo sapiens |