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Literature summary for 1.14.11.2 extracted from

  • Kivirikko, K.I.; Myllylä, R.
    Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes (1982), Methods Enzymol., 82, 245-304.
    View publication on PubMed
Search for more literature for 1.14.11.2

General Stability

General Stability Organism
labile in tissue extracts Gallus gallus
labile in tissue extracts Homo sapiens
labile in tissue extracts Rattus norvegicus
stabilization by NaCl, detergents Gallus gallus
stabilization by NaCl, detergents Homo sapiens
stabilization by NaCl, detergents Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000001
-
 Protocollagen
-
 Gallus gallus
0.00000001
-
 Protocollagen
-
 Homo sapiens
0.00000001
-
 Protocollagen
-
 Rattus norvegicus
0.01 0.03 (Pro-Pro-Gly)10
-
 Homo sapiens
0.01 0.03 (L-Pro-L-Pro-Gly)10
-
 Gallus gallus
0.01 0.03 (L-Pro-L-Pro-Gly)10
-
 Rattus norvegicus
0.1 0.5 (L-Pro-L-Pro-Gly)5
-
 Gallus gallus
0.1 0.5 (L-Pro-L-Pro-Gly)5
-
 Homo sapiens
0.1 0.5 (L-Pro-L-Pro-Gly)5
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum cisternae Gallus gallus 5783
-
endoplasmic reticulum cisternae Homo sapiens 5783
-
endoplasmic reticulum cisternae Rattus norvegicus 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Gallus gallus
Fe2+
-
 Homo sapiens
Fe2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000 240000 gel filtration Homo sapiens
230000 240000 gel filtration Rattus norvegicus
230000 240000 sedimentation equilibrium centrifugation Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
glycoprotein subunit alpha: contains 16 residues of mannose, 1 residue of galactose and at least 2 residues of N-acetylglucosamine, subunit beta: contains 2 residues of mannose and 3 residues of galactose Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
of the beta-subunit using ammonium sulfate fractionation, chromatography on preimmune IgG column and immunoadsorbent column, and gel filtration Rattus norvegicus
using affinity chromatography on a column containing a polypeptide substrate of the enzyme linked to agarose, elution of the enzyme with a second peptide substrate and separation of the enzyme from this peptide by gel filtration Gallus gallus
using affinity chromatography on a column containing a polypeptide substrate of the enzyme linked to agarose, elution of the enzyme with a second peptide substrate and separation of the enzyme from this peptide by gel filtration Rattus norvegicus
using affinity chromatography on a column containing poly-(L-proline) linked to agarose, elution with the same polypeptide of a lower molecular weight, and gel filtration Gallus gallus
using affinity chromatography on a column containing poly-(L-proline) linked to agarose, elution with the same polypeptide of a lower molecular weight, and gel filtration Homo sapiens
using affinity chromatography on a column containing poly-(L-proline) linked to agarose, elution with the same polypeptide of a lower molecular weight, and gel filtration Rattus norvegicus
using fractionating precipitation, ion-exchange chromatography and gel filtration Gallus gallus
using fractionating precipitations, ion-exchange chromatographies and gel filtrations Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-
skin
-
 Gallus gallus
-
skin
-
 Homo sapiens
-
skin new born Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method Gallus gallus
additional information
-
 assay method Homo sapiens
additional information
-
 assay method Rattus norvegicus
additional information
-
 specific activity of the purified beta-subunit Rattus norvegicus
1.3
-
 purified enzyme Homo sapiens
1.3
-
 purified enzyme Rattus norvegicus
1.5
-
 purified enzyme Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Gallus gallus (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Rattus norvegicus (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Homo sapiens (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Rattus norvegicus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Homo sapiens ? + succinate + CO2
-
 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Rattus norvegicus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
proline containing peptide + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
proline containing peptide + 2-oxoglutarate + O2
-
 Homo sapiens 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
proline containing peptide + 2-oxoglutarate + O2
-
 Rattus norvegicus 4-hydroxyproline containing peptide + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Gallus gallus
tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Homo sapiens
tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Gallus gallus
7.4
-
-
 Homo sapiens
7.4
-
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Gallus gallus
ascorbate
-
 Homo sapiens
ascorbate
-
 Rattus norvegicus