Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bleomycin | activation | Gallus gallus | |
bleomycin | activation | Homo sapiens | |
bovine serum albumin | activation | Gallus gallus | |
bovine serum albumin | activation | Homo sapiens | |
catalase | activation | Gallus gallus | |
catalase | activation | Homo sapiens | |
Chelating agents | activation | Gallus gallus | |
Chelating agents | activation | Homo sapiens | |
dithiothreitol | activation | Gallus gallus | |
dithiothreitol | activation | Homo sapiens | |
nucleoside triphosphates | stimulate | Gallus gallus | |
nucleoside triphosphates | stimulate | Homo sapiens | |
Thymol | activation | Gallus gallus | |
Thymol | activation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Gallus gallus | |
2,2'-dipyridyl | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | luminal site | Gallus gallus | 5783 | - |
endoplasmic reticulum | luminal site | Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus | |
Fe2+ | purified enzyme does not require exogenous Fe2+ to obtain full enzymic activity, the iron in the holomeric placental enzyme appears to be more tightly bound than in the chick embryo enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | 600000 | value depending on salt concentration of buffer | Gallus gallus |
400000 | 600000 | value depending on salt concentration of buffer | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | Gallus gallus | key enzyme in collagen biosynthesis, catalyzes the conversion of selected prolyl residues to trans-hydroxyproline in nascent or completed pro-alpha chains of procollagen | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | Homo sapiens | key enzyme in collagen biosynthesis, catalyzes the conversion of selected prolyl residues to trans-hydroxyproline in nascent or completed pro-alpha chains of procollagen | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate composition | Gallus gallus |
glycoprotein | carbohydrate composition | Homo sapiens |
glycoprotein | 29.8 residues mannose and 3.8 residues N-acetyl-glucosamine per 240 kDa | Homo sapiens |
glycoprotein | 35.4 residues mannose and 3.9 residues N-acetyl-glucosamine per 240 kDa | Gallus gallus |
Purification (Comment) | Organism |
---|---|
using chromatography on DEAE-cellulose column, affinity column, anion-exchange chromatography and a second chromatography on DEAE-cellulose column | Gallus gallus |
using chromatography on DEAE-cellulose column, affinity column, anion-exchange chromatography and a second chromatography on DEAE-cellulose column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | key enzyme in collagen biosynthesis, catalyzes the conversion of selected prolyl residues to trans-hydroxyproline in nascent or completed pro-alpha chains of procollagen | Gallus gallus | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | key enzyme in collagen biosynthesis, catalyzes the conversion of selected prolyl residues to trans-hydroxyproline in nascent or completed pro-alpha chains of procollagen | Homo sapiens | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Homo sapiens | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure of the tetramer, physicochemical properties of the subunits | Gallus gallus |
More | structure of the tetramer, physicochemical properties of the subunits | Homo sapiens |
tetramer | alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE | Gallus gallus |
tetramer | alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE | Homo sapiens |
tetramer | alpha: 64000, beta: 62000, ratio 1 to 2 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Gallus gallus | |
2-oxoglutarate | - |
Homo sapiens | |
ascorbate | requirement | Gallus gallus | |
ascorbate | requirement | Homo sapiens |