Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | powerful inhibitor at 1 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley strain | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley strain | - |
Purification (Comment) | Organism |
---|---|
separation of 4-hydroxylase activity from 3-hydroxylase activity using affinity chromatography and gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Rattus norvegicus | ? | - |
? | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Rattus norvegicus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Rattus norvegicus Sprague-Dawley | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |