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Literature summary for 1.14.11.2 extracted from

  • Kuutti, L.R.; Tuderman, L.; Kivirikko, K.I.
    Human prolyl hydroxylase. Purification, partial characterization and preparation of antiserum to the enzyme (1975), Eur. J. Biochem., 57, 181-188.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
2 * 61000 + 2 * 640000 Homo sapiens
230000 240000 gel filtration Homo sapiens
640000
-
2 * 61000 + 2 * 640000 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity chromatography on a column containing poly-(L-proline) linked to agarose, elution with the same polypeptide of a lower molecular weight, and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fetus skin, mixture of fetal tissues Homo sapiens
-
skin fetus Homo sapiens
-
skin fetus, adult Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.02 1.38 purified enzyme Homo sapiens
77.8
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
procollagen L-proline + 2-oxoglutarate + O2
-
Homo sapiens procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 61000 + 2 * 640000 Homo sapiens