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Literature summary for 1.14.11.2 extracted from

  • Tanaka, M.; Shibata, H.; Uchida, T.
    A new prolyl hydroxylase acting on poly-L-proline, from suspension cultured cells of Vinca rosea (1980), Biochim. Biophys. Acta, 616, 188-198.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Catharanthus roseus
dithiothreitol activation Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
additional information high concentrations of salts are inhibitory Catharanthus roseus
NaCl more than 0.3 M Catharanthus roseus
poly-L-hydroxyproline MW: 30000, inhibitory Catharanthus roseus
potassium phosphate
-
 Catharanthus roseus
Sodium acetate
-
 Catharanthus roseus
succinate 11.5% inhibition at 0.5 mM and 38.3% inhibition at 3 mM Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 O2
-
 Catharanthus roseus
0.23
-
 poly(L-proline)
-
 Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome 40.7% of total activity Catharanthus roseus
-
-
mitochondrion 47.9% of total activity Catharanthus roseus 5739
-
soluble 11.4% of total activity Catharanthus roseus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Catharanthus roseus
-

Storage Stability

Storage Stability Organism
-80°C, dialyzed against 25% glycerol, 1 month Catharanthus roseus
4°C, unstable Catharanthus roseus
freeze-thawing, unstable Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 5,10, poor substrates Catharanthus roseus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
Gly-Pro8 + 2-oxoglutarate + O2 96% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Catharanthus roseus ?
-
 ?
octa-L-proline + 2-oxoglutarate + O2 81.8% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 MW 2000, 6000 and 12000 Catharanthus roseus poly(4-hydroxyproline) + succinate + CO2
-
 ?
poly(L-proline) + 2-oxoglutarate + O2
-
 Catharanthus roseus poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 91.4% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate Km: 0.09 mM Catharanthus roseus
ascorbate
-
 Catharanthus roseus
additional information alpha-ketoglutarate cannot be replaced by pyruvate and oxaloacetate Catharanthus roseus