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Literature summary for 1.14.11.2 extracted from

  • Gorres, K.L.; Raines, R.T.
    Direct and continuous assay for prolyl 4-hydroxylase (2009), Anal. Biochem., 386, 181-185.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.2

Inhibitors

Inhibitors Comment Organism Structure
2,4-pyridine dicarboxylate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Homo sapiens
0.5
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, fluoride-based assay Homo sapiens
1.2
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, HPLC-based assay Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ P4H is a nonheme iron dioxygenase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2 Homo sapiens
-
 procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,4S)-4-fluoroproline + 2-oxoglutarate + O2 development and evaluation of an assay method that continuously and directly detects turnover of the proline-containing substrate. The assay is based on (2S,4S)-4-fluoroproline, a proline analogue that is transformed into (2S)-4-oxoproline and inorganic fluoride by P4H using a fluoride ion-selective electrode for detection Homo sapiens (2S)-4-oxoproline + fluoride
-
 ?
PEG-Gly-Tyr-4-fluoroproline-GlyOEt + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
procollagen L-proline + 2-oxoglutarate + O2
-
 Homo sapiens procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2 prolyl 4-hydroxylase is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands Homo sapiens procollagen trans-(2S,4R)-4-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
P4H
-
 Homo sapiens
prolyl 4-hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, fluoride-based assay Homo sapiens
6
-
 PEG-Gly-Tyr-4-fluoroproline-GlyOEt pH 7.8, 22°C, recombinant enzyme, HPLC-based assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function P4H is a nonheme iron dioxygenase that catalyzes the posttranslational hydroxylation of (2S)-Pro residues in protocollagen strands. The resulting (2S,4R)-4-hydroxyproline residues are essential for the folding, secretion, and stability of the collagen triple helix Homo sapiens