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Literature summary for 1.14.11.2 extracted from

  • Song, W.; Yang, C.; Zhu, C.; Morris, P.; Zhang, X.
    Crystal structure and expression patterns of prolyl 4-hydroxylases from Phytophthora capsici (2019), Biochem. Biophys. Res. Commun., 508, 1011-1017 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
ascorbate mainly stimulats P4HA1 Mus musculus
LY83583 might increase the enzymatic activity of prolyl 4-hydroxylases to increase type I collagen maturation. LY83583 activated prolyl 4-hydrolases differed from ascorbate-activated prolyl 4-hydroxylase in two aspects: (1) LY83583 activats both P4HA1 and P4HA2 involved in collagen maturation whereas ascorbate mainly stimulats P4HA1 in collagen maturation, (2) LY83583 does not induce N259 glycosylation on P4HA1 as ascorbate does Mus musculus
LY83583 might increase the enzymatic activity of prolyl 4-hydroxylases to increase type I collagen maturation. LY83583 activated prolyl 4-hydrolases differs from ascorbate-activated prolyl 4-hydroxylase in two aspects: (1) LY83583 activats both P4HA1 and P4HA2 involved in collagen maturation whereas ascorbate mainly stimulats P4HA1 in collagen maturation, (2) LY83583 does not induce N259 glycosylation on P4HA1 as ascorbate does Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) pLysS cells Phytophthora capsici

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method Phytophthora capsici

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Phytophthora capsici

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2 Mus musculus
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2 Phytophthora capsici
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2 Phytophthora capsici LT1534
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q60715 prolyl 4-hydroxylase subunit alpha-1
-
Mus musculus Q60716 prolyl 4-hydroxylase subunit alpha-2
-
Phytophthora capsici A0A4P1LYH8
-
-
Phytophthora capsici LT1534 A0A4P1LYH8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N259 glycosylation Mus musculus

Purification (Commentary)

Purification (Comment) Organism
-
 Phytophthora capsici

Source Tissue

Source Tissue Comment Organism Textmining
embryonic fibroblast
-
 Mus musculus
-
mycelium
-
 Phytophthora capsici
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2
-
 Mus musculus procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2
-
 Phytophthora capsici procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2 the binding affinity of 2-oxoglutarate to PcP4H1 is very low Phytophthora capsici procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2
-
 Phytophthora capsici LT1534 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2 the binding affinity of 2-oxoglutarate to PcP4H1 is very low Phytophthora capsici LT1534 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
P4ha1
-
 Mus musculus
P4ha2
-
 Mus musculus
prolyl 4-hydroxylase
-
 Mus musculus
prolyl 4-hydroxylase
-
 Phytophthora capsici

Expression

Organism Comment Expression
Phytophthora capsici in mycelia, all P4Hs are downregulated in response to hypoxia, but the expression of PcP4H1 is most affected. Pc4H1 is upregulated more than 110fold at the onset of infection, and Pc4H5 is upregulated 7fold, while the expression of other P4Hs is unchanged up

General Information

General Information Comment Organism
malfunction knockout of either P4ha1 or P4ha2 greatly reduces LY83583-stimulated type I collagen maturation whereas silencing of both P4ha1 and P4ha2 completely blocks LY83583-induced type I collagen maturation Mus musculus
physiological function prolyl 4-hydroxylase play central roles in the collagen stabilization, hypoxia sensing, and translational regulation in eukaryotes Phytophthora capsici