Literature summary for 1.14.11.2 extracted from

Shi, J.; Ma, X.; Gao, Y.; Fan, D.; Zhu, C.; Mi, Y.; Xue, W.
Hydroxylation of human type III collagen alpha chain by recombinant coexpression with a viral prolyl 4-hydroxylase in Escherichia coli (2017), Protein J., 36, 322-331 .

Search for more literature for 1.14.11.2

Application

Application	Comment	Organism
synthesis	viral P4H (A085R) is coexpressed as a soluble and active monomer in Escherichia coli with full-length human collagen alpha1(III) chain (COL3A1). This coexpression system can be used for the production of hydroxylated human collagen alpha1(III) chain (COL3A1) in Escherichia coli. The method avoids the complexity of P4H assembly	Paramecium bursaria Chlorella virus 1

Cloned(Commentary)

Cloned (Comment)	Organism
expression by Escherichia coli BL21(DE3)	Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
procollagen L-proline + 2-oxoglutarate + O2	Paramecium bursaria Chlorella virus 1	-	procollagen trans-4-hydroxy-L-proline + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Paramecium bursaria Chlorella virus 1	Q84406	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
procollagen L-proline + 2-oxoglutarate + O2	-	Paramecium bursaria Chlorella virus 1	procollagen trans-4-hydroxy-L-proline + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
A085R	-	Paramecium bursaria Chlorella virus 1
prolyl 4-hydroxylase A085R	-	Paramecium bursaria Chlorella virus 1

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Release 2023.1 (January 2023)