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Literature summary for 1.14.11.21 extracted from

  • Paradkar, A.S.; Jensen, S.E.
    Functional analysis of the gene encoding the clavaminate synthase 2 isoenzyme involved in clavulanic acid biosynthesis in Streptomyces clavuligerus (1995), J. Bacteriol., 177, 1307-1314.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
isozymes CAS1 and CAS2 encoded by genes cas1 and cas2, respectively, genetic analysis Streptomyces clavuligerus

Protein Variants

Protein Variants Comment Organism
additional information construction of a disruption mutant of isozyme cas2 by a gene replacement procedure, mutant shows no activity in starch-asparagine medium, but reduced activity, compared to the wild-type, in soy medium which is due to CAS1 isozyme, different regulation of the 2 isozymes concerning nutritional conditions Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus this reaction is due to a proclavaminate amidinohydrolase, i.e. cla amidinoproclavaminate + succinate + CO2 + H2O
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus NRRL 3585 this reaction is due to a proclavaminate amidinohydrolase, i.e. cla amidinoproclavaminate + succinate + CO2 + H2O
-
?
dihydroclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
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clavaminate + succinate + CO2 + H2O
-
?
additional information Streptomyces clavuligerus enzyme plays a critical role in the biosynthesis of the clavam metabolites ?
-
?
additional information Streptomyces clavuligerus biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor ?
-
?
additional information Streptomyces clavuligerus NRRL 3585 enzyme plays a critical role in the biosynthesis of the clavam metabolites ?
-
?
additional information Streptomyces clavuligerus NRRL 3585 biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor ?
-
?
proclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
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dihydroclavaminate + succinate + CO2 + H2O
-
?
proclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus NRRL 3585
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dihydroclavaminate + succinate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
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-
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Streptomyces clavuligerus
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2 isozymes CAS1 and CAS2, encoded by genes cas1 and cas2, respectively
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Streptomyces clavuligerus NRRL 3585
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-
-

Reaction

Reaction Comment Organism Reaction ID
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 this reaction is due to a proclavaminate amidinohydrolase PAH, gene cla Streptomyces clavuligerus
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of wild-type and mutant in different growth media Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 this reaction is due to a proclavaminate amidinohydrolase, i.e. cla Streptomyces clavuligerus amidinoproclavaminate + succinate + CO2 + H2O
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 this reaction is due to a proclavaminate amidinohydrolase, i.e. cla Streptomyces clavuligerus NRRL 3585 amidinoproclavaminate + succinate + CO2 + H2O
-
?
dihydroclavaminate + 2-oxoglutarate + O2
-
Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
?
dihydroclavaminate + 2-oxoglutarate + O2 cyclization Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
?
additional information enzyme plays a critical role in the biosynthesis of the clavam metabolites Streptomyces clavuligerus ?
-
?
additional information biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor Streptomyces clavuligerus ?
-
?
additional information enzyme plays a critical role in the biosynthesis of the clavam metabolites Streptomyces clavuligerus NRRL 3585 ?
-
?
additional information biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor Streptomyces clavuligerus NRRL 3585 ?
-
?
proclavaminate + 2-oxoglutarate + O2
-
Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
?
proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
?
proclavaminate + 2-oxoglutarate + O2
-
Streptomyces clavuligerus NRRL 3585 dihydroclavaminate + succinate + CO2 + H2O
-
?
proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus NRRL 3585 dihydroclavaminate + succinate + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
CAS
-
Streptomyces clavuligerus