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Literature summary for 1.14.11.21 extracted from

  • Zhang, Z.; Ren, J.s.; Harlos, K.; McKinnon, C.H.; Clifton, I.J.; Schofield, C.J.
    Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centred rearrangements (2002), FEBS Lett., 517, 7-12.
    View publication on PubMed
Search for more literature for 1.14.11.21

Crystallization (Commentary)

Crystallization (Comment) Organism
anaerobically, recombinant isozyme CAS1, in complex with Fe2+, 2-oxoglutarate and deoxyguanidinoproclavaminate, hanging drop vapour diffusion method, protein solution: 20 mg/ml, reservoir solution: 0.1 M Tris, pH 7.5, 2 M ammonium sulfate, 10% glycerol, 20°C, treatment with NO under anaerobic conditions, structure determination and analysis Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ conformation during catalytic reaction Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 clavaminate + succinate + CO2 + H2O
-
 ?
additional information Streptomyces clavuligerus biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor ?
-
 ?
proclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 dihydroclavaminate + succinate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus Q05581 recombinant CAS1
-
Streptomyces clavuligerus Q05581 crystal structure of isozyme CAS1, complexed or uncomplexed, recombinant from Escherichia coli, Protein Data Bank: 1DS0, 1DS1, 1DRY, 1DRT, 1GVG
-

Reaction

Reaction Comment Organism Reaction ID
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 reaction mechanism Streptomyces clavuligerus
a
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2 cyclization Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
additional information biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor Streptomyces clavuligerus ?
-
 ?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 isozyme CAS1 Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
proclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
 ?
proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
 ?