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Literature summary for 1.14.11.26 extracted from

  • An, Y.; Dong, H.; Liu, G.
    Expression of cefF significantly decreased deacetoxycephalosporin C formation during cephalosporin C production in Acremonium chrysogenum (2012), J. Ind. Microbiol. Biotechnol., 39, 269-274.
    View publication on PubMed
Search for more literature for 1.14.11.26

Cloned(Commentary)

Cloned (Comment) Organism
gene cefEF Acremonium chrysogenum
gene cefF, subcloning in Escherichia coli, functional expression in Acremonium chrysogenum strain CGMCC3.3795 via Agrobacterium tumefaciens-mediated transformation under the promoter of pcbC, leading to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Acremonium chrysogenum
Fe2+ required Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deacetoxycephalosporin C + 2-oxoglutarate + O2 Acremonium chrysogenum
-
 deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2 Acremonium chrysogenum CGMCC3.3795
-
 deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2 Streptomyces clavuligerus CGMCC4.1611
-
 deacetylcephalosporin C + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum P11935 formerly Cephalosporium acremonium, gene cefEF
-
Acremonium chrysogenum CGMCC3.3795 P11935 formerly Cephalosporium acremonium, gene cefEF
-
Streptomyces clavuligerus P42220 gene cefF
-
Streptomyces clavuligerus CGMCC4.1611 P42220 gene cefF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
 Acremonium chrysogenum deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
 Acremonium chrysogenum CGMCC3.3795 deacetylcephalosporin C + succinate + CO2
-
 ?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus CGMCC4.1611 deacetylcephalosporin C + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
Cephalosporin biosynthesis expandase/hydroxylase UniProt Acremonium chrysogenum
DAOC synthase
-
 Streptomyces clavuligerus
DAOC/DAC synthase
-
 Acremonium chrysogenum

General Information

General Information Comment Organism
metabolism the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains Acremonium chrysogenum
metabolism two enzymes, deacetoxycephalosporin C synthase and deacetoxycephalosporin C hydroxylase, are involved in the conversion of penicillin N into deacetylcephalosporin C in Streptomyces clavuligerus Streptomyces clavuligerus
additional information additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview Acremonium chrysogenum