Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | stimulation | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of Flag-tagged protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
H305A | more than 90% loss of activity | Saccharomyces cerevisiae |
additional information | overexpression results in moderate decrease in dimethyl-histone 3 L-lysine 36 and a slight decrease in trimethyl-histone 3 L-lysine 36 along with unaltered methyl-histone 3 L-lysine 36 levels. Deletion of the N-terminal PHD domain leads to about 50% decrease in activity. Deletion of C-terminal 125, 193, or 244 amino acids results in more than 90% loss of activity | Saccharomyces cerevisiae |
T302A | more than 90% loss of activity | Saccharomyces cerevisiae |
Y315A | more than 90% loss of activity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ is required | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
x * 56500, calculated | Saccharomyces cerevisiae |
250000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40034 | histone demethylase Jhd1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethyl-histone 3 L-lysine 36 + 2-oxoglutarate + O2 | - |
Saccharomyces cerevisiae | methyl-histone 3 L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
additional information | no substrate: methyl-histone 3 L-lysine 4, histone 3 L-lysine 79 | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 56500, calculated | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Jhd1 | - |
Saccharomyces cerevisiae |
JmjC domain-containing histone demethylase 1 | - |
Saccharomyces cerevisiae |