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Literature summary for 1.14.11.28 extracted from

  • Mori, H.; Shibasaki, T.; Uozaki, Y.; Ochiai, K.; Ozaki, A.
    Detection of novel proline 3-hydroxylase activities in Streptomyces and Bacillus spp. by regio- and stereospecific hydroxylation of L-proline (1996), Appl. Environ. Microbiol., 62, 1903-1907.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.28

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 82% inhibition at 0.1 mM, 98% inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C Streptomyces sp.
CuSO4 59% inhibition at 0.1 mM, complete inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C Streptomyces sp.
EDTA 95% inhibition at 2 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C Streptomyces sp.
ZnSO4 95% inhibition at 0.1 mM, complete inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM added to cell extracts, 60% inhibition without addition of Fe2+ Bacillus sp. (in: Bacteria)
Fe2+ 1 mM added to cell extracts, 60% inhibition without addition of Fe2+ Streptomyces sp.
Fe2+ 1 mM added to cell extracts, 85% inhibition without addition of Fe2+ Bacillus sp. (in: Bacteria)
Fe2+ 1 mM added to cell extracts, complete inhibition without addition of Fe2+ Streptomyces canus

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) TH2
-
-
-
Bacillus sp. (in: Bacteria) TH3
-
-
-
Streptomyces canus
-
 strain ATCC 12647
-
Streptomyces sp.
-
-
-
Streptomyces sp. TH1
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Bacillus sp. (in: Bacteria)
-
cell culture
-
 Streptomyces sp.
-
cell culture
-
 Streptomyces canus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00029
-
-
 Streptomyces canus
0.00119
-
 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C Streptomyces sp.
0.00132
-
-
 Bacillus sp. (in: Bacteria)
0.00149
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2-oxoglutarate + O2
-
 Bacillus sp. (in: Bacteria) cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces canus cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Bacillus sp. (in: Bacteria) TH2 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Bacillus sp. (in: Bacteria) TH3 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
proline 3-hydroxylase
-
 Bacillus sp. (in: Bacteria)
proline 3-hydroxylase
-
 Streptomyces sp.
proline 3-hydroxylase
-
 Streptomyces canus

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate strictly required, highest activity at 4 mM Bacillus sp. (in: Bacteria)
2-oxoglutarate strictly required, highest activity at 4 mM Streptomyces sp.
2-oxoglutarate strictly required, highest activity at 4 mM Streptomyces canus