Cloned (Comment) | Organism |
---|---|
AlkB is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
isoforms ABH2 and ABH3 are expressed in baculovirus-infected Sf9 insect cells | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Escherichia coli | |
Fe2+ | dependent on | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Escherichia coli |
Ni-NTA agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | 3-methylthymine residues in DNA is repaired inefficiently in vivo. The ABH3 protein repairs 3-methylthymine in both single-stranded and double-stranded polydeoxynucleotides, whereas the ABH2 protein prefers a duplex substrate | Homo sapiens | DNA-thymine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | 3-methylthymine residues in DNA is repaired inefficiently in vivo. The AlkB protein repairs 3-methylthymine in both single-stranded and double-stranded polydeoxynucleotidese | Escherichia coli | DNA-thymine + formaldehyde + succinate + CO2 | - |
? | |
methylated poly(deoxythymine) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(deoxythymine) + formaldehyde + succinate + CO2 | - |
? | |
methylated poly(deoxythymine) + 2-oxoglutarate + O2 | - |
Homo sapiens | poly(deoxythymine) + formaldehyde + succinate + CO2 | - |
? | |
additional information | the enzyme does not demethylate 3-methyladenine, 7-methyladenine, 7-methylguanine, thymine (5-methyluracil), and 5-methylcytosine | Escherichia coli | ? | - |
? | |
additional information | the enzyme does not demethylate 3-methyladenine, 7-methyladenine, 7-methylguanine, thymine (5-methyluracil), and 5-methylcytosine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABH2 | isoform | Homo sapiens |
ABH3 | isoform | Homo sapiens |
AlkB | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
repair of DNA-3-methylthymine by AlkB is optimal at pH 6.0, but inefficient | Escherichia coli |
6.5 | - |
repair of DNA-3-methylthymine by ABH3 is optimal at pH 6.5, but inefficient | Homo sapiens |
7.6 | 8 | DNA-3-methylcytosine is repaired optimally at pH 7.5-8.0 | Escherichia coli |
7.6 | 8 | DNA-3-methylcytosine is repaired optimally at pH 7.5-8.0 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli | |
2-oxoglutarate | - |
Homo sapiens |