Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Homo sapiens | |
Fe2+ | required for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05050 | - |
- |
Homo sapiens | Q6NS38 | isoform ABH2 | - |
Homo sapiens | Q96Q83 | isoform ABH3 | - |
Purification (Comment) | Organism |
---|---|
His-tagged AlkB is purified by Ni-NTA agarose column chromatography, untagged Alkb is purified by DEAE-cellulose column chromatography, Sepharose column chromatography, and Mono S column chromatography | Escherichia coli |
His-tagged enzyme is purified by Ni-NTA agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-ethyladenine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-adenine + acetaldehyde + succinate + CO2 | - |
ir | |
DNA-1-ethyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + acetaldehyde + succinate + CO2 | - |
ir | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | good substrate | Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | good substrate | Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-1-methylguanine + 2-oxoglutarate + O2 | weak substrate | Homo sapiens | DNA-guanine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-1-methylguanine + 2-oxoglutarate + O2 | weak substrate | Escherichia coli | DNA-guanine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | good substrate | Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | good substrate | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | weak substrate | Homo sapiens | DNA-thymine + formaldehyde + succinate + CO2 | - |
ir | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | weak substrate | Escherichia coli | DNA-thymine + formaldehyde + succinate + CO2 | - |
ir | |
methylated poly(deoxyadenine) + 2-oxoglutarate + O2 | - |
Homo sapiens | poly(deoxyadenine) + formaldehyde + succinate + CO2 | - |
ir | |
methylated poly(deoxyadenine) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(deoxyadenine) + formaldehyde + succinate + CO2 | - |
ir | |
methylated poly(deoxycytosine) + 2-oxoglutarate + O2 | - |
Homo sapiens | poly(deoxycytosine) + formaldehyde + succinate + CO2 | - |
ir | |
methylated poly(deoxycytosine) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(deoxycytosine) + formaldehyde + succinate + CO2 | - |
ir | |
additional information | AlkB binds to single-stranded DNA, but less efficiently to double-stranded DNA | Escherichia coli | ? | - |
? | |
additional information | isoform ABH2 shows some preference for methylated poly(dA) over methylated poly(dC) and also prefers a double-stranded substrate. not ABH2 cannot demethylate 1-methyladenine in RNA | Homo sapiens | ? | - |
? | |
additional information | isoform ABH2 shows some preference for methylated poly(dC) over methylated poly(dA) and also prefers a single-stranded substrate. Isoform ABH3 can also demethylate 1-methyladenine in RNA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABH2 | isoform | Homo sapiens |
ABH3 | isoform | Homo sapiens |
AlkB | - |
Homo sapiens |
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Homo sapiens | |
2-oxoglutarate | - |
Escherichia coli |