Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.33 extracted from

  • Ougland, R.; Zhang, C.M.; Liiv, A.; Johansen, R.F.; Seeberg, E.; Hou, Y.M.; Remme, J.; Falnes, P.O.
    AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation (2004), Mol. Cell, 16, 107-116.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells. ABH3 expressed in insect cells contains no posttranslational modifications but is nevertheless found to be 5fold more active than ABH3 purified from Escherichia coli, suggesting ABH3 may not be correctly folded when purified from Escherichia coli Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Escherichia coli
Fe2+ required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
methylated luciferase-mRNA + 2-oxoglutarate + O2
-
 Homo sapiens luciferase-mRNA + formaldehyde + succinate + CO2
-
 ?
methylated luciferase-mRNA + 2-oxoglutarate + O2 AlkB is used at a maximal concentration of 0.00083 mM for this reaction since the luciferase-mRNA is degraded when higher AlkB concentrations are used Escherichia coli luciferase-mRNA + formaldehyde + succinate + CO2
-
 ?
methylated tRNA-Phe + 2-oxoglutarate + O2
-
 Escherichia coli tRNA-Phe + formaldehyde + succinate + CO2
-
 ?
methylated tRNA-Phe + 2-oxoglutarate + O2
-
 Homo sapiens tRNAPhe + formaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ABH3
-
 Homo sapiens
AlkB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Escherichia coli
2-oxoglutarate
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function ABH3 restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with ABH3 prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by ABH3 Homo sapiens
physiological function AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation. A methylation-induced block in translation of an mRNA can be readily relieved by treatment with AlkB prior to translation. Chemical methylation of tRNA-Phe inhibits aminoacylation and translation, but the inhibition can be reversed by AlkB Escherichia coli