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Literature summary for 1.14.11.33 extracted from

  • Aas, P.A.; Otterlei, M.; Falnes, P.O.; Vagbo, C.B.; Skorpen, F.; Akbari, M.; Sundheim, O.; Bjoras, M.; Slupphaug, G.; Seeberg, E.; Krokan, H.E.
    Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA (2003), Nature, 421, 859-863.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Escherichia coli
Fe2+ required for activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylated double-stranded bacteriophage lambda + 2-oxoglutarate + O2 Homo sapiens
-
 double-stranded bacteriophage lambda + formaldehyde + succinate + CO2
-
 ?
methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 Homo sapiens isoform ABH3 reactivates methylated single-stranded RNA bacteriophage MS2 RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
additional information Homo sapiens isoform ABH2 does not reactivate methylated RNA bacteriophage MS2 or methylated double-stranded bacteriophage lambda ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Homo sapiens Q96Q83 isoform ABH3
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and UnoS column chromatography Escherichia coli
Ni-NTA column chromatography and UnoS column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
methylated double-stranded bacteriophage lambda + 2-oxoglutarate + O2
-
 Homo sapiens double-stranded bacteriophage lambda + formaldehyde + succinate + CO2
-
 ?
methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 isoform ABH3 reactivates methylated single-stranded RNA bacteriophage MS2 Homo sapiens RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
additional information isoform ABH2 does not reactivate methylated RNA bacteriophage MS2 or methylated double-stranded bacteriophage lambda Homo sapiens ?
-
 ?
additional information ABH2 does not repair RNA. ABH2 acts more efficiently on double-stranded DNA Homo sapiens ?
-
 ?
additional information ABH3 also repairs RNA. ABH3 prefers single-stranded nucleic acids Homo sapiens ?
-
 ?
additional information AlkB also repairs RNA. AlkB prefers single-stranded nucleic acids. Escherichia coli ?
-
 ?
additional information repair of alkylated poly(A) by ABH3 is almost as efficient as repair of poly(dA), and repair of poly(C) is substantially more efficient than repair of poly(dA), although not as good as repair of poly(dC) and single-stranded bacteriophage M13 DNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ABH2 isoform Homo sapiens
ABH3 isoform Homo sapiens
AlkB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Escherichia coli
2-oxoglutarate
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function isoform ABH2 repairs DNA close to the replication forks, is more active on double-stranded than on single-stranded DNA and is present in different subnuclear compartments at different stages of the cell cycle Homo sapiens
physiological function the nuclear localization of ABH3 and the clear preference for single-stranded DNA and RNA suggest that ABH3 has a role in maintenance of nuclear single-stranded DNA and RNA, with genes undergoing transcription potentially being its main targets Homo sapiens