Cloned (Comment) | Organism |
---|---|
AlkB overexpression in Escherichia coli AlkB-deficient strains HK82 and BW25113. AlkB mutant strain HK82 is transformed with the low-copy-number expression plasmid pJB658 containing the alkB gene following a toluic acid-inducible promoter. The recombinant AlkB expressed is less active than the natural endogenous form | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli | - |
DNA-base + formaldehyde + succinate + CO2 | - |
? | |
DNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli AB1157 | - |
DNA-base + formaldehyde + succinate + CO2 | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | Escherichia coli | - |
adenine + formaldehyde + succinate + CO2 | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | Escherichia coli AB1157 | - |
adenine + formaldehyde + succinate + CO2 | - |
? | |
N3-methylcytosine + 2-oxoglutarate + O2 | Escherichia coli | - |
cytosine + formaldehyde + succinate + CO2 | - |
? | |
N3-methylcytosine + 2-oxoglutarate + O2 | Escherichia coli AB1157 | - |
cytosine + formaldehyde + succinate + CO2 | - |
? | |
RNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli | - |
RNA-base + formaldehyde + succinate + CO2 | - |
? | |
RNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli AB1157 | - |
RNA-base + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli AB1157 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-base + formaldehyde + succinate + CO2 | - |
? | |
DNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli AB1157 | DNA-base + formaldehyde + succinate + CO2 | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | adenine + formaldehyde + succinate + CO2 | - |
? | |
N1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli AB1157 | adenine + formaldehyde + succinate + CO2 | - |
? | |
N3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | cytosine + formaldehyde + succinate + CO2 | - |
? | |
N3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli AB1157 | cytosine + formaldehyde + succinate + CO2 | - |
? | |
RNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli | RNA-base + formaldehyde + succinate + CO2 | - |
? | |
RNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli AB1157 | RNA-base + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | an AlkB-deficient strain accumulates N1-methyladenine at a high rate | Escherichia coli |
additional information | the recombinant AlkB expressed is less active than the natural endogenous form | Escherichia coli |
physiological function | protein AlkB repairs cytotoxic 1-methyladenine and 3-methylcytosine lesions induced by methyl methanesulfonate in DNA and RNA in vitro and in pre-damaged DNA and RNA bacteriophages in vivo, quantification of AlkB-mediated repair of endogenous RNA and DNA in vivo, overview | Escherichia coli |