BRENDA - Enzyme Database
show all sequences of 1.14.11.35

Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis

You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.; J. Am. Chem. Soc. 128, 6566-6567 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.57
-
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
Streptomyces avermitilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
non-heme iron, alpha-ketoglutarate-dependent hydroxylase
Streptomyces avermitilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37121
-
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
37139
-
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces avermitilis
Q82IZ1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(+/-)-1-deoxypentalenic acid + O2
-
719644
Streptomyces avermitilis
11beta-hydroxy-1-deoxypentalenic acid + succinate + CO2
-
-
-
?
additional information
no substrate: (+/-)-pentalenene, (+/-)-pentalen-13-ol
719644
Streptomyces avermitilis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
Synonyms
Synonyms
Commentary
Organism
ptlH
-
Streptomyces avermitilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2
-
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
Streptomyces avermitilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.57
-
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
Streptomyces avermitilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
non-heme iron, alpha-ketoglutarate-dependent hydroxylase
Streptomyces avermitilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37121
-
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
37139
-
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(+/-)-1-deoxypentalenic acid + O2
-
719644
Streptomyces avermitilis
11beta-hydroxy-1-deoxypentalenic acid + succinate + CO2
-
-
-
?
additional information
no substrate: (+/-)-pentalenene, (+/-)-pentalen-13-ol
719644
Streptomyces avermitilis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37121, calculated, x * 37139, MALDI-TOF
Streptomyces avermitilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2
-
(+/-)-1-deoxypentalenic acid
pH 6.0, temperature not specified in the publication
Streptomyces avermitilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Streptomyces avermitilis
Other publictions for EC 1.14.11.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719845
You
Crystal structure of the non-h ...
Streptomyces avermitilis
J. Biol. Chem.
282
36552-36560
2007
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719644
You
Pentalenolactone biosynthesis. ...
Streptomyces avermitilis
J. Am. Chem. Soc.
128
6566-6567
2006
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1
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1
2
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1
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