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Literature summary for 1.14.11.4 extracted from

  • Armstrong, L.C.; Last, J.A.
    Rat lysyl hydroxylase: molecular cloning, mRNA distribution and expression in a baculovirus system (1995), Biochim. Biophys. Acta, 1264, 93-102.
    View publication on PubMed
Search for more literature for 1.14.11.4

Application

Application Comment Organism
medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Homo sapiens
medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells using a baculovirus vector Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Procollagen L-lysine
-
 Rattus norvegicus
0.106
-
 2-oxoglutarate
-
 Rattus norvegicus
0.122
-
 ascorbate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 x * 85000, SDS-PAGE and Western blotting Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus Q63321
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
 specific activity of the recombinant enzyme three-fold higher than the specific activity of native enzyme Gallus gallus
additional information
-
 specific activity of the recombinant enzyme three-fold higher than the specific activity of native enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + O2 + ascorbate
-
 Rattus norvegicus succinate + CO2 + dehydroascorbate + H2O
-
 ?
peptidyl-L-lysine + 2-oxoglutarate + O2
-
 Gallus gallus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
peptidyl-L-lysine + 2-oxoglutarate + O2
-
 Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
peptidyl-L-lysine + 2-oxoglutarate + O2
-
 Rattus norvegicus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
[procollagen] L-lysine + 2-oxoglutarate + O2
-
 Rattus norvegicus [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 r

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE and Western blotting Rattus norvegicus