Literature summary for 1.14.11.4 extracted from

  • Takaluoma, K.; Lantto, J.; Myllyharju, J.
    Lysyl hydroxylase 2 is a specific telopeptide hydroxylase, while all three isoenzymes hydroxylate collagenous sequences (2007), Matrix Biol., 26, 396-403.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in H5 insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
type IV procollagen isozyme LH3, at 37°C Homo sapiens
0.1
-
type I procollagen isozyme LH2, at 37°C Homo sapiens
0.3
-
type IV procollagen isozyme LH1, at 37°C Homo sapiens
0.31
-
(Ile-Lys-Gly)3 isozyme LH2, at 37°C Homo sapiens
0.04
-
type IV procollagen isozyme LH2, at 37°C Homo sapiens
0.23
-
type I procollagen isozyme LH1, at 37°C Homo sapiens
0.08
-
type I procollagen isozyme LH3, at 37°C Homo sapiens
0.34
-
(Ile-Lys-Gly)3 isozyme LH3, at 37°C Homo sapiens
0.47
-
(Ile-Lys-Gly)3 isozyme LH1, at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ProBond metal chelate affinity resin chromatography and Chelating Sepharose fast flow column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information LH2 hydroxylates the N telopeptide of the full-length procollagen alpha type I chain when coexpressed with this isoenzyme Homo sapiens ?
-
?
collagen + 2-oxoglutarate + O2
-
Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
?
(Ile-Lys-Gly)3 + 2-oxoglutarate + O2
-
Homo sapiens ?
-
?
type IV procollagen + 2-oxoglutarate + O2
-
Homo sapiens ?
-
?
type I procollagen + 2-oxoglutarate + O2
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
lysyl hydroxylase
-
Homo sapiens
LH2
-
Homo sapiens
LH3
-
Homo sapiens
LH1
-
Homo sapiens