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Literature summary for 1.14.11.43 extracted from

  • Müller, T.A.; Zavodszky, M.I.; Feig, M.; Kuhn, L.A.; Hausinger, R.P.
    Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases (2006), Protein Sci., 15, 1356-1368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.43

Protein Variants

Protein Variants Comment Organism
E69A exhibits 30.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 42% compared to the wild-type value Sphingobium herbicidovorans
G97I/N98G exhibits 3.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.6% compared to the wild-type value Sphingobium herbicidovorans
G97N/N98G exhibits 0.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate Sphingobium herbicidovorans
H208A exhibits about 1.4% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.1% compared to the wild-type value Sphingobium herbicidovorans
H272A exhibits about 1.2% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate Sphingobium herbicidovorans
Q162F exhibits about 4.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate Sphingobium herbicidovorans
R207A exhibits about 17.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 2% compared to the wild-type value Sphingobium herbicidovorans
R207V exhibits about 2.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate Sphingobium herbicidovorans
R274A inactive mutant enzyme Sphingobium herbicidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme E69A Sphingobium herbicidovorans
0.161
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, wild-type enzyme Sphingobium herbicidovorans
1.1
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme G97I/N98G Sphingobium herbicidovorans
2.5
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme R207A Sphingobium herbicidovorans
2.9
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme H208A Sphingobium herbicidovorans

Organism

Organism UniProt Comment Textmining
Sphingobium herbicidovorans Q700X4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium herbicidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-dichlorprop. RdpA exclusively transforms the (S)-enantiomer of dichlorprop Sphingobium herbicidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
 ?
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-mecoprop. RdpA exclusively transforms the (S)-enantiomer of mecoprop Sphingobium herbicidovorans 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
 ?
additional information construction of homology models of SdpA and RdpA from Sphingomonas herbicidovorans MH and the use of docking to identify residues likely to be involved in herbicide binding Sphingobium herbicidovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
SdpA
-
 Sphingobium herbicidovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sphingobium herbicidovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme H208A Sphingobium herbicidovorans
0.7
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme G97I/N98G Sphingobium herbicidovorans
3.97
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme R207A Sphingobium herbicidovorans
4
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme E69A Sphingobium herbicidovorans
16.83
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, wild-type enzyme Sphingobium herbicidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Sphingobium herbicidovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme H208A Sphingobium herbicidovorans
0.64
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme G97I/N98G Sphingobium herbicidovorans
1.59
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme R207A Sphingobium herbicidovorans
93.96
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, mutant enzyme E69A Sphingobium herbicidovorans
104.5
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C, wild-type enzyme Sphingobium herbicidovorans