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Literature summary for 1.14.11.47 extracted from

  • Sekirnik, R.; Wilkins, S.; Bush, J.; Tarhonskaya, H.; Münzel, M.; Hussein, A.; Flashman, E.; Mohammed, S.; McDonough, M.; Loenarz, C.; Schofield, C.
    YcfDRM is a thermophilic oxygen-dependent ribosomal protein uL16 oxygenase (2018), Extremophiles, 22, 553-562 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
 2-oxoglutarate pH 7.5, 37°C Rhodothermus marinus
0.075
-
 [50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 pH 7.5, 37°C Rhodothermus marinus
0.1
-
 O2 pH 7.5, 37°C Rhodothermus marinus
0.208
-
 [50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg pH 7.5, 37°C Rhodothermus marinus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe(II)-coordinating triad consists of H133, D135, H196. Km value at 37°C is 0.0146 mM Rhodothermus marinus

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus
-
-
-
Rhodothermus marinus DSM 4252
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme displays a temperature-dependent variation in uL16 hydroxylation. Dioxygen availability can be a limiting factor for ycfD catalysis at high temperatures, consistent with incomplete uL16 hydroxylation observed Rhodothermus marinus ?
-
-
additional information enzyme displays a temperature-dependent variation in uL16 hydroxylation. Dioxygen availability can be a limiting factor for ycfD catalysis at high temperatures, consistent with incomplete uL16 hydroxylation observed Rhodothermus marinus DSM 4252 ?
-
-
[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 + 2-oxoglutarate + O2
-
 Rhodothermus marinus [50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
-
 ?
[50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 + 2-oxoglutarate + O2
-
 Rhodothermus marinus DSM 4252 [50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
-
 ?
[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg + 2-oxoglutarate + O2
-
 Rhodothermus marinus [50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-(3R)-3-hydroxy-L-Arg + succinate + CO2
-
 ?
[50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg + 2-oxoglutarate + O2
-
 Rhodothermus marinus DSM 4252 [50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-(3R)-3-hydroxy-L-Arg + succinate + CO2
-
 ?
[50S ribosomal protein L16]-L-Arg82 + 2-oxoglutarate + O2
-
 Rhodothermus marinus [50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
-
 ?
[50S ribosomal protein L16]-L-Arg82 + 2-oxoglutarate + O2
-
 Rhodothermus marinus DSM 4252 [50S ribosomal protein L16]-(3R)-3-hydroxy-L-Arg82 + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ycfD
-
 Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
84
-
 melting temperature Rhodothermus marinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
 [50S ribosomal cyclic peptide 6KKPAEVRMGKGKG88-linker]-L-Arg82 pH 7.5, 37°C Rhodothermus marinus
0.5
-
 [50S ribosomal peptide KPVTKKPAEVRMGKGKGSVE-NH2]-L-Arg pH 7.5, 37°C Rhodothermus marinus