BRENDA - Enzyme Database show
show all sequences of 1.14.11.51

DNA methylation on N-adenine in mammalian embryonic stem cells

Wu, T.P.; Wang, T.; Seetin, M.G.; Lai, Y.; Zhu, S.; Lin, K.; Liu, Y.; Byrum, S.D.; Mackintosh, S.G.; Zhong, M.; Tackett, A.; Wang, G.; Hon, L.S.; Fang, G.; Swenberg, J.A.; Xiao, A.Z.; Nature 532, 329-333 (2016)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D233A
reduced activity of the recombinant mutant protein
Mus musculus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains Fe2+
Mus musculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43746
-
x * 43746, calculated from sequence
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6-methyladenine in DNA + 2-oxoglutarate + O2
Mus musculus
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
adenine in DNA + formaldehyde + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
P0CB42
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
embryonic stem cell
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6-methyladenine in DNA + 2-oxoglutarate + O2
-
736883
Mus musculus
adenine in DNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in DNA + 2-oxoglutarate + O2
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
736883
Mus musculus
adenine in DNA + formaldehyde + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43746, calculated from sequence
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D233A
reduced activity of the recombinant mutant protein
Mus musculus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains Fe2+
Mus musculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43746
-
x * 43746, calculated from sequence
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6-methyladenine in DNA + 2-oxoglutarate + O2
Mus musculus
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
adenine in DNA + formaldehyde + succinate + CO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
embryonic stem cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6-methyladenine in DNA + 2-oxoglutarate + O2
-
736883
Mus musculus
adenine in DNA + formaldehyde + succinate + CO2
-
-
-
?
N6-methyladenine in DNA + 2-oxoglutarate + O2
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
736883
Mus musculus
adenine in DNA + formaldehyde + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43746, calculated from sequence
Mus musculus
General Information
General Information
Commentary
Organism
physiological function
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons
Mus musculus
Other publictions for EC 1.14.11.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736883
Wu
DNA methylation on N-adenine i ...
Mus musculus
Nature
532
329-333
2016
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1
-
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1
1
1
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2
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1
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2
1
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1
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1
1
1
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1
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2
1
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1
1
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742207
Zhou
DNA N6-methyladenine demethyl ...
Homo sapiens
Bone Res.
4
16033
2016
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1
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1
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1
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1
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1
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1
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1
2
2
1
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738025
Zhang
N6-methyladenine DNA modificat ...
Drosophila melanogaster
Cell
161
893-906
2015
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1
1
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4
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2
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1
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1
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2
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1
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1
1
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742235
Greer
DNA methylation on N6-adenine ...
Caenorhabditis elegans
Cell
161
868-878
2015
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-
-
-
1
-
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1
-
3
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1
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1
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1
1
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738971
Ye
-
Repair of methyl lesions in RN ...
Homo sapiens
MedChemComm
5
1797-1803
2014
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1
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1
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