Crystallization (Comment) | Organism |
---|---|
enzyme shows a stretch-out conformation of its Flip1 motif, explaining the observed preference of ALKBH1 for unpairing substrates, in which the flipped N6-mA is primed for catalysis. Structures of ALKBH1 bound to a 21-mer bulged DNA show the need of both flanking duplexes and a flipped base for recognition and catalysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K158A | mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 | Mus musculus |
R159A | mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 | Mus musculus |
R160A | mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 | Mus musculus |
R167A | mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 | Mus musculus |
R169A | mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 | Mus musculus |
S235D | mutation disrupts N6-mA demethylation activity | Mus musculus |
S235E | mutation disrupts N6-mA demethylation activity | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P0CB42 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ALKBH1 prefers bubbled or bulged DNAs as substrate. The middle N6-mA in the 3-nt bulge is efficiently demethylated compared to that in the 3-nt bubble (97% vs. 18% demethylation fraction) and the 1st and 3rd N6-mAs are more resistant to demethylation. N6-mAs deposited in the flexible region of hairpin (DNA stem loop), D-loop and R-loop are efficiently demethylated as in bubble and bulge, while those in cruciform and replication fork are less demethylated. m6rA in RNA hairpin, RNA-RNA bubble and RNA-DNA hybrid bubble can also be demethylated by ALKBH1, as well as 5-methylcytosine at anticodon loop and 1-methyladenosine | Mus musculus | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
ALKBH1 | - |
Mus musculus |
General Information | Comment | Organism |
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metabolism | N6-hydroxymethyladenine exists as an oxidized intermediate during N6-mA demethylation by ALKBH1 | Mus musculus |