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Literature summary for 1.14.11.54 extracted from
- Switching demethylation activities between AlkB family RNA/DNA demethylases through exchange of active-site residues (2014), Angew. Chem. Int. Ed. Engl., 53, 3659-3662 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D135I | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
| D135N | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
| E136L | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00232 | - |
RNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 0.00276 | - |
RNA-N6-methyladenine | mutant enzyme D135I, pH and temperature not specified in the publication | Escherichia coli | |
| 0.00347 | - |
RNA-N6-methyladenine | mutant enzyme E136L, pH and temperature not specified in the publication | Escherichia coli | |
| 0.00577 | - |
RNA-N6-methyladenine | mutant enzyme D135N, pH and temperature not specified in the publication | Escherichia coli | |
| 0.01493 | - |
RNA-N6-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P05050 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-N1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | RNA-adenine + formaldehyde + succinate + CO2 | - |
? |  |
| RNA-N6-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | RNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlkB | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
RNA-N6-methyladenine | mutant enzyme D135I, pH and temperature not specified in the publication | Escherichia coli | |
| 0.0017 | - |
RNA-N6-methyladenine | mutant enzyme D135N, pH and temperature not specified in the publication | Escherichia coli | |
| 0.0017 | - |
RNA-N6-methyladenine | mutant enzyme E136L, pH and temperature not specified in the publication | Escherichia coli | |
| 0.0018 | - |
RNA-N6-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 0.062 | - |
RNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
RNA-N6-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
| 0.3 | - |
RNA-N6-methyladenine | mutant enzyme D135N, pH and temperature not specified in the publication | Escherichia coli | |
| 0.5 | - |
RNA-N6-methyladenine | mutant enzyme E136L, pH and temperature not specified in the publication | Escherichia coli | |
| 0.58 | - |
RNA-N6-methyladenine | mutant enzyme D135I, pH and temperature not specified in the publication | Escherichia coli | |
| 26.7 | - |
RNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
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