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Literature summary for 1.14.11.54 extracted from

  • Chen, Z.; Qi, M.; Shen, B.; Luo, G.; Wu, Y.; Li, J.; Lu, Z.; Zheng, Z.; Dai, Q.; Wang, H.
    Transfer RNA demethylase ALKBH3 promotes cancer progression via induction of tRNA-derived small RNAs (2019), Nucleic Acids Res., 47, 2533-2545 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.54

Protein Variants

Protein Variants Comment Organism
L177A mutation at the active site, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo Homo sapiens
R122A mutant is defective in its DNA/RNA binding groove, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96Q83
-
-

Source Tissue

Source Tissue Comment Organism Textmining
DU-145 cell
-
 Homo sapiens
-
HEK-293T cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
Hep-G2 cell
-
 Homo sapiens
-
PC-3 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-methyladenine in tRNA + 2-oxoglutarate + O2
-
 Homo sapiens adenine in tRNA + formaldehyde + succinate + CO2
-
 ?
N3-methylcytidine in tRNA + 2-oxoglutarate + O2
-
 Homo sapiens cytidine in tRNA + formaldehyde + succinate + CO2
-
 ?

General Information

General Information Comment Organism
physiological function ALKBH3 is a 1-methyladenosine and 3-methylcytidine demethylase of tRNA. ALKBH3 can promote cancer cell proliferation, migration and invasion. The m1A demethylated tRNA is more sensitive to angiogenin cleavage, followed by generating tRNA-derived small RNAs around the anticodon regions. in ALKBH3-/- HeLa cells, m1A level of tRNA in cytoplasm, but not in nucleus, is remarkably higher than that of wild type cells. Deletion of ALKBH3 significantly increases the m1A levels of most target tRNAs such as tRNAGluCTC, tRNASeCTCA, tRNAGlyGCC, tRNAHisGTG, tRNASerGCT, tRNAGlnCTG, tRNAValCAC, tRNAArgCCT, tRNALeuCAA, tRNAGlyCCC, tRNAAlaCGC and tRNAeMetCAT. m1A levels of some targets, such as tRNALysCTT and tRNAAsnGTT, are not affected. m3C levels of target tRNA are significantly upregulated in Alkbh3-/- HeLa cells Homo sapiens